Detailed information of TA system TA226628

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 483921..484453
• Replicon: plasmid pTA1_2
• Accession: NZ_CP088106
• Organism: Rhizobium ruizarguesonis strain TA1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: RlegTA1_RS30600
• Protein ID: WP_027688710.1
• Coordinates: 483921..484214 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: RlegTA1_RS30605
• Protein ID: WP_024320231.1
• Coordinates: 484211..484453 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RlegTA1_RS30580 (RlegTA1_30580)	479653..480696	-	1044	WP_018070765.1	ABC transporter permease	-
RlegTA1_RS30585 (RlegTA1_30585)	480696..482213	-	1518	WP_018070764.1	ATP-binding cassette domain-containing protein	-
RlegTA1_RS30590 (RlegTA1_30590)	482210..483208	-	999	WP_018070763.1	LacI family DNA-binding transcriptional regulator	-
RlegTA1_RS30595 (RlegTA1_30595)	483407..483847	+	441	WP_018070762.1	RbsD/FucU domain-containing protein	-
RlegTA1_RS30600 (RlegTA1_30600)	483921..484214	-	294	WP_027688710.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RlegTA1_RS30605 (RlegTA1_30605)	484211..484453	-	243	WP_024320231.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
RlegTA1_RS30610 (RlegTA1_30610)	484580..485482	-	903	WP_027688709.1	MBL fold metallo-hydrolase	-
RlegTA1_RS30615 (RlegTA1_30615)	485585..486217	-	633	WP_027688708.1	DsbA family protein	-
RlegTA1_RS30620 (RlegTA1_30620)	486332..487216	+	885	WP_017992594.1	LysR family transcriptional regulator	-
RlegTA1_RS30625 (RlegTA1_30625)	487266..487655	+	390	WP_231286872.1	acylphosphatase	-
RlegTA1_RS30630 (RlegTA1_30630)	487798..488451	+	654	WP_024320234.1	Rieske (2Fe-2S) protein	-
RlegTA1_RS30635 (RlegTA1_30635)	488448..489293	+	846	WP_018070754.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd	1..662287	662287

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11275.76 Da
• Isoelectric Point: 4.9134

>T226628 WP_027688710.1 NZ_CP088106:c484214-483921 [Rhizobium ruizarguesonis]
MSFKLSAEAEDDIIAIAEQSVRIFGAGQAKRYHDELFALFDLIAVNPRFARERDEIDPPVRIHPFKAHLIVYRIEDDETI
FVIRIRHAHEDWASDSI

Nucleotide

• Length: 294 bp

>T226628 NZ_CP122587:324444-324812 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8881.03 Da
• Isoelectric Point: 9.7765

>AT226628 WP_024320231.1 NZ_CP088106:c484453-484211 [Rhizobium ruizarguesonis]
MATMNVSLPGPMKDWVEAQARTGRYSNASDYVRDLIRRDQTRNDKIAAMQSFVEAGLQSGVGIRSKDELFAEAMARANKS

Nucleotide

• Length: 243 bp

>AT226628 NZ_CP122587:324226-324447 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA