Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 871674..872328 Replicon chromosome
Accession NC_010468
Organism Escherichia coli ATCC 8739

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag ECOLC_RS04325 Protein ID WP_000244777.1
Coordinates 871921..872328 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ECOLC_RS04320 Protein ID WP_000354046.1
Coordinates 871674..871940 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECOLC_RS04295 866843..867586 + 744 WP_000951964.1 SDR family oxidoreductase -
ECOLC_RS04300 867643..869076 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
ECOLC_RS04305 869121..869432 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
ECOLC_RS04310 869596..870255 + 660 WP_000250274.1 hemolysin III family protein -
ECOLC_RS04315 870451..871431 - 981 WP_000886083.1 tRNA-modifying protein YgfZ -
ECOLC_RS04320 871674..871940 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ECOLC_RS04325 871921..872328 + 408 WP_000244777.1 toxin CptA Toxin
ECOLC_RS04330 872368..872889 - 522 WP_001055874.1 flavodoxin FldB -
ECOLC_RS04335 873001..873897 + 897 WP_000806628.1 site-specific tyrosine recombinase XerD -
ECOLC_RS04340 873922..874632 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ECOLC_RS04345 874638..876371 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T22656 WP_000244777.1 NC_010468:871921-872328 [Escherichia coli ATCC 8739]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T22656 NC_010468:871921-872328 [Escherichia coli ATCC 8739]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT22656 WP_000354046.1 NC_010468:871674-871940 [Escherichia coli ATCC 8739]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT22656 NC_010468:871674-871940 [Escherichia coli ATCC 8739]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References