Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5498500..5499125 Replicon chromosome
Accession NZ_CP087978
Organism Klebsiella variicola subsp. variicola strain ZH07

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag LPW14_RS26615 Protein ID WP_008807903.1
Coordinates 5498500..5498883 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LPW14_RS26620 Protein ID WP_004150355.1
Coordinates 5498883..5499125 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPW14_RS26600 (LPW14_26600) 5495866..5496768 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LPW14_RS26605 (LPW14_26605) 5496765..5497400 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
LPW14_RS26610 (LPW14_26610) 5497397..5498326 + 930 WP_055323209.1 formate dehydrogenase accessory protein FdhE -
LPW14_RS26615 (LPW14_26615) 5498500..5498883 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
LPW14_RS26620 (LPW14_26620) 5498883..5499125 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LPW14_RS26625 (LPW14_26625) 5499330..5500247 + 918 WP_032733990.1 alpha/beta hydrolase -
LPW14_RS26630 (LPW14_26630) 5500262..5501203 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
LPW14_RS26635 (LPW14_26635) 5501248..5501685 - 438 WP_012543288.1 D-aminoacyl-tRNA deacylase -
LPW14_RS26640 (LPW14_26640) 5501682..5502542 - 861 WP_008807907.1 virulence factor BrkB family protein -
LPW14_RS26645 (LPW14_26645) 5502536..5503135 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T226515 WP_008807903.1 NZ_CP087978:c5498883-5498500 [Klebsiella variicola subsp. variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T226515 NZ_CP122503:c4284804-4284454 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT226515 WP_004150355.1 NZ_CP087978:c5499125-5498883 [Klebsiella variicola subsp. variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT226515 NZ_CP122503:c4285049-4284798 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References