Detailed information of TA system TA226513

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4456313..4457040
• Replicon: chromosome
• Accession: NZ_CP087978
• Organism: Klebsiella variicola subsp. variicola strain ZH07

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A979H205
• Locus tag: LPW14_RS21790
• Protein ID: WP_000854674.1
• Coordinates: 4456675..4457040 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: LPW14_RS21785
• Protein ID: WP_072413956.1
• Coordinates: 4456313..4456654 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPW14_RS21750 (LPW14_21750)	4451467..4452342	+	876	WP_072422522.1	GTPase family protein	-
LPW14_RS21755 (LPW14_21755)	4452586..4453302	+	717	WP_059305023.1	WYL domain-containing protein	-
LPW14_RS21760 (LPW14_21760)	4453338..4453790	+	453	WP_048998898.1	hypothetical protein	-
LPW14_RS21765 (LPW14_21765)	4453862..4454335	+	474	Protein_4279	hypothetical protein	-
LPW14_RS21770 (LPW14_21770)	4454455..4455276	+	822	WP_048998885.1	DUF932 domain-containing protein	-
LPW14_RS21775 (LPW14_21775)	4455307..4455747	+	441	WP_048998884.1	antirestriction protein	-
LPW14_RS21780 (LPW14_21780)	4455760..4456302	+	543	WP_072413955.1	DNA repair protein RadC	-
LPW14_RS21785 (LPW14_21785)	4456313..4456654	+	342	WP_072413956.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LPW14_RS21790 (LPW14_21790)	4456675..4457040	+	366	WP_000854674.1	TA system toxin CbtA family protein	Toxin
LPW14_RS21795 (LPW14_21795)	4457483..4460128	-	2646	WP_164875709.1	LuxR C-terminal-related transcriptional regulator	-
LPW14_RS21800 (LPW14_21800)	4460501..4461460	+	960	WP_002889884.1	thiamine pyrophosphate-dependent dehydrogenase E1 component subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4440754..4466223	25469

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13593.61 Da
• Isoelectric Point: 8.0503

>T226513 WP_000854674.1 NZ_CP087978:4456675-4457040 [Klebsiella variicola subsp. variicola]
MKTLPATISRAAEPGLSPVGVWQTLLTRLMEQHYGLTLNDTPFSDETVIKEHIDAGITLANAINFLVEKYELVRIDRNGF
TSQVQAPYLTATDILHARRACGLMTRFSYREVSNIVLSRSR

Nucleotide

• Length: 366 bp

>T226513 NZ_CP122503:3846857-3847135 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12669.32 Da
• Isoelectric Point: 6.6214

>AT226513 WP_072413956.1 NZ_CP087978:4456313-4456654 [Klebsiella variicola subsp. variicola]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPRH
AHCVTLYHNGFACEADTLGSCGYVYIAVYPSQR

Nucleotide

• Length: 342 bp

>AT226513 NZ_CP122503:3846594-3846854 [Escherichia coli]
ATGGCTGCAAATGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA