Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1870801..1871391 Replicon chromosome
Accession NZ_CP087978
Organism Klebsiella variicola subsp. variicola strain ZH07

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag LPW14_RS09060 Protein ID WP_008804165.1
Coordinates 1871059..1871391 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag LPW14_RS09055 Protein ID WP_012541132.1
Coordinates 1870801..1871058 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPW14_RS09035 (LPW14_09035) 1866411..1866986 + 576 WP_016161434.1 hypothetical protein -
LPW14_RS09040 (LPW14_09040) 1867163..1867999 + 837 WP_230648945.1 alpha/beta hydrolase -
LPW14_RS09045 (LPW14_09045) 1868205..1869176 + 972 WP_230648947.1 sensor domain-containing diguanylate cyclase -
LPW14_RS09050 (LPW14_09050) 1869173..1870273 - 1101 WP_117105472.1 AarF/UbiB family protein -
LPW14_RS09055 (LPW14_09055) 1870801..1871058 + 258 WP_012541132.1 antitoxin Antitoxin
LPW14_RS09060 (LPW14_09060) 1871059..1871391 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LPW14_RS09070 (LPW14_09070) 1871649..1873085 - 1437 WP_016161429.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
LPW14_RS09080 (LPW14_09080) 1873523..1874977 - 1455 WP_008804170.1 AMP nucleosidase -
LPW14_RS09085 (LPW14_09085) 1875108..1875353 - 246 WP_008804171.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T226501 WP_008804165.1 NZ_CP087978:1871059-1871391 [Klebsiella variicola subsp. variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T226501 NZ_CP122503:204337-204444 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAACGGAAGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT226501 WP_012541132.1 NZ_CP087978:1870801-1871058 [Klebsiella variicola subsp. variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT226501 NZ_CP122503:c204288-204225 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References