Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 2203973..2204421 Replicon chromosome
Accession NC_010296
Organism Microcystis aeruginosa NIES-843

Toxin (Protein)


Gene name hicA Uniprot ID B0JHA8
Locus tag MAE_RS10645 Protein ID WP_012265576.1
Coordinates 2204227..2204421 (-) Length 65 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID I4GA01
Locus tag MAE_RS10640 Protein ID WP_002771759.1
Coordinates 2203973..2204230 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAE_RS10620 2199390..2200019 - 630 WP_041804020.1 Uma2 family endonuclease -
MAE_RS10625 2200054..2202102 - 2049 WP_012265575.1 DNA phosphorothioation-associated putative methyltransferase -
MAE_RS10630 2202146..2202925 - 780 WP_002796685.1 photosystem II S4 domain protein -
MAE_RS10635 2202997..2203506 - 510 WP_002796683.1 hypothetical protein -
MAE_RS10640 2203973..2204230 - 258 WP_002771759.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MAE_RS10645 2204227..2204421 - 195 WP_012265576.1 type II toxin-antitoxin system HicA family toxin Toxin
MAE_RS10650 2204725..2205945 - 1221 WP_002796679.1 efflux RND transporter periplasmic adaptor subunit -
MAE_RS10655 2205989..2206210 - 222 WP_002737463.1 DUF4327 family protein -
MAE_RS10660 2206379..2207266 - 888 WP_012265577.1 acetylglutamate kinase -
MAE_RS10665 2207346..2208569 - 1224 WP_012265578.1 amidohydrolase -
MAE_RS33775 2208652..2209131 - 480 WP_162467772.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(6-62)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7280.49 Da        Isoelectric Point: 10.7019

>T22628 WP_012265576.1 NC_010296:c2204421-2204227 [Microcystis aeruginosa NIES-843]
MKVRDVLKRLEADGWYQIRMRGSHRILAHETKPGIVVVPGKSGDDIPKGTLASIWKQAQLEDKR

Download         Length: 195 bp

>T22628 NC_010296:c2204421-2204227 [Microcystis aeruginosa NIES-843]
TTGAAAGTTCGTGATGTACTCAAACGATTAGAAGCTGATGGTTGGTATCAGATCCGAATGCGCGGGAGCCATCGTATCCT
TGCCCATGAAACAAAGCCAGGAATCGTGGTTGTTCCTGGCAAGTCTGGGGACGACATACCGAAAGGAACATTAGCATCGA
TTTGGAAACAAGCTCAATTGGAGGATAAACGATGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9297.57 Da        Isoelectric Point: 3.9826

>AT22628 WP_002771759.1 NC_010296:c2204230-2203973 [Microcystis aeruginosa NIES-843]
MKEYAVIYEQGETNWGAMVPDLPGCVSIGDTFEEVQENIKEAIELYLEVLQERGETIPEPTTKVGLVGVAAGGFLPTTQK
LLGFH

Download         Length: 258 bp

>AT22628 NC_010296:c2204230-2203973 [Microcystis aeruginosa NIES-843]
ATGAAAGAGTATGCCGTCATTTACGAACAAGGCGAAACAAACTGGGGAGCAATGGTTCCCGACTTGCCCGGTTGCGTGAG
TATTGGAGATACCTTTGAAGAGGTTCAGGAAAATATTAAAGAAGCGATTGAACTTTACCTTGAAGTTTTGCAAGAACGCG
GGGAAACCATTCCAGAACCCACAACTAAAGTCGGATTGGTTGGAGTCGCTGCAGGAGGGTTCTTGCCGACTACCCAAAAA
CTGCTGGGGTTTCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9JIH3

Antitoxin

Source ID Structure
AlphaFold DB I4GA01

References