Detailed information of TA system TA022612

Bioinformatically predicted

Overview

TA module

• Type: III
• Classification (family/domain): toxIN/ToxN(toxin)
• Location: 47183..47913
• Replicon: plasmid pBWB402
• Accession: NC_010181
• Organism: Bacillus weihenstephanensis KBAB4

Toxin (Protein)

• Gene name: toxN
• Uniprot ID: A9VVE1
• Locus tag: BCERKBAB4_RS28345
• Protein ID: WP_012260000.1
• Coordinates: 47341..47913 (+)
• Length: 191 a.a.

Antitoxin (RNA)

Gene name	toxI
Locus tag	-
Coordinates	47183..47279 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCERKBAB4_RS28315	42300..43070	-	771	Protein_43	hypothetical protein	-
BCERKBAB4_RS28320	43216..43491	-	276	WP_080515484.1	hypothetical protein	-
BCERKBAB4_RS28325	43683..45113	-	1431	Protein_45	IS4 family transposase	-
BCERKBAB4_RS28335	45438..45902	+	465	WP_080019825.1	metal-dependent hydrolase	-
BCERKBAB4_RS28340	46068..47093	+	1026	WP_002144419.1	DUF3991 and TOPRIM domain-containing protein	-
-	47183..47279	+	97	NuclAT_0	-	Antitoxin
-	47183..47279	+	97	NuclAT_0	-	Antitoxin
-	47183..47279	+	97	NuclAT_0	-	Antitoxin
-	47183..47279	+	97	NuclAT_0	-	Antitoxin
BCERKBAB4_RS28345	47341..47913	+	573	WP_012260000.1	type III toxin-antitoxin system ToxN/AbiQ family toxin	Toxin
BCERKBAB4_RS28350	48068..50212	+	2145	WP_002144421.1	type IA DNA topoisomerase	-
BCERKBAB4_RS28355	50283..50699	+	417	WP_002144422.1	CPBP family intramembrane metalloprotease	-
BCERKBAB4_RS28360	51339..51797	-	459	WP_040963227.1	hypothetical protein	-
BCERKBAB4_RS28365	52129..52581	+	453	WP_002144427.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..75107	75107

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ToxN/AbiQ toxin (8-171)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 22270.58 Da
• Isoelectric Point: 9.1859

>T22612 WP_012260000.1 NC_010181:47341-47913 [Bacillus mycoides KBAB4]
MANKDNPKFYTISTKYIDYLRETDSKVPFNKDEQHSRPYVGVLEKINGHDYFVPLTSRNDKNLNSQVSVKLFDIDNPEKR
IGVLLVNNMIPVPEKECKEIDIAEKTETDPKYGNLMLKQYLFLKENMDRVSNKVEKVYKDVTVQGKPSQKQKFLKGVCCD
FSKLEEKCQEYKEKGQARRIAYMPQMGRER

Nucleotide

• Length: 573 bp

>T22612 NC_010181:47341-47913 [Bacillus mycoides KBAB4]
TTGGCTAATAAAGATAATCCTAAATTTTACACTATTTCAACCAAATATATAGACTATCTAAGAGAAACAGATAGTAAAGT
ACCCTTTAATAAAGACGAACAGCATAGCAGACCGTATGTAGGTGTATTGGAAAAAATCAATGGTCATGATTATTTTGTGC
CTTTAACATCACGTAATGATAAAAATCTTAATAGCCAGGTCAGTGTAAAGTTATTCGATATTGATAACCCTGAGAAGCGA
ATTGGAGTTTTATTGGTCAATAACATGATTCCAGTCCCAGAAAAAGAGTGTAAAGAAATTGATATAGCAGAAAAAACGGA
GACAGATCCTAAGTATGGTAATTTGATGTTAAAACAGTATTTGTTTTTAAAAGAGAATATGGATCGAGTTTCTAATAAAG
TGGAAAAAGTATATAAAGATGTAACTGTACAAGGAAAGCCTTCACAAAAACAGAAATTCTTAAAAGGAGTTTGCTGTGAT
TTCTCTAAATTAGAGGAAAAGTGCCAGGAATATAAAGAAAAGGGTCAAGCTAGAAGAATAGCATATATGCCCCAAATGGG
GCGTGAGAGATAA

Antitoxin

Nucleotide

• Length: 97 bp

>AT22612 NC_010181:47183-47279 [Bacillus mycoides KBAB4]
GTAGCCTTACTTTAGTAGGCGCGAAAAAATTGGTGTAACTCTACCGTAGTGAGTACCAAAAAATTGGTGTAGCCTTACCG
TAGTAGGCACAAAAAAA

Structures

Toxin

• AlphaFold DB: A9VVE1

Antitoxin