Detailed information of TA system TA226053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 46340..46881
• Replicon: chromosome
• Accession: NZ_CP087696
• Organism: Xylella taiwanensis strain PLS16B

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Z9JGT1
• Locus tag: LPH57_RS00305
• Protein ID: WP_038273166.1
• Coordinates: 46558..46881 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Z9JGL9
• Locus tag: LPH57_RS00300
• Protein ID: WP_038273164.1
• Coordinates: 46340..46561 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPH57_RS00250 (LPH57_00250)	41916..42152	-	237	WP_230428212.1	major capsid protein	-
LPH57_RS00255 (LPH57_00255)	42161..42367	-	207	WP_038273075.1	hypothetical protein	-
LPH57_RS00260 (LPH57_00260)	42370..42684	-	315	WP_230428211.1	hypothetical protein	-
LPH57_RS00265 (LPH57_00265)	42960..43547	-	588	WP_038273076.1	hypothetical protein	-
LPH57_RS00270 (LPH57_00270)	43744..43887	-	144	WP_160165233.1	hypothetical protein	-
LPH57_RS00275 (LPH57_00275)	44007..44207	-	201	WP_038273077.1	hypothetical protein	-
LPH57_RS00280 (LPH57_00280)	44256..44519	-	264	WP_162814095.1	hypothetical protein	-
LPH57_RS00285 (LPH57_00285)	44523..44684	-	162	WP_160165236.1	hypothetical protein	-
LPH57_RS00290 (LPH57_00290)	44974..45303	+	330	WP_230428286.1	DUF3693 domain-containing protein	-
LPH57_RS00295 (LPH57_00295)	45392..45892	-	501	Protein_58	tyrosine-type recombinase/integrase	-
LPH57_RS00300 (LPH57_00300)	46340..46561	+	222	WP_038273164.1	antitoxin MazE family protein	Antitoxin
LPH57_RS00305 (LPH57_00305)	46558..46881	+	324	WP_038273166.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LPH57_RS00310 (LPH57_00310)	46982..48130	-	1149	WP_069636227.1	zonular occludens toxin domain-containing protein	-
LPH57_RS00315 (LPH57_00315)	48130..48471	-	342	WP_038273089.1	DUF2523 family protein	-
LPH57_RS00320 (LPH57_00320)	48475..49542	-	1068	WP_152536670.1	virulence factor TspB C-terminal domain-related protein	-
LPH57_RS00325 (LPH57_00325)	50010..50228	-	219	WP_051482398.1	major capsid protein	-
LPH57_RS00330 (LPH57_00330)	50237..50446	-	210	WP_230428208.1	hypothetical protein	-
LPH57_RS00335 (LPH57_00335)	50446..50568	-	123	WP_230430865.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	28063..54115	26052

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (9-72)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11807.97 Da
• Isoelectric Point: 9.1188

>T226053 WP_038273166.1 NZ_CP087696:46558-46881 [Xylella taiwanensis]
MTQRGDLVTVSLQGDYGKPRPALILQSDLLMELDSVVLCPVTSELRNAIFRITVEPNIANGLRTLSQIMVDKISTLPRSK
ISKPFGHLNDERIKAVEKALLLIVGII

Nucleotide

• Length: 324 bp

>T226053 NZ_CP121766:3160568-3161146 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAGCACAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAGGCCGCGCACCCGGACAAGGGCGAACAGGAACGCGCCGACCTGTTGCAGTTGACCCGTCACCTCGACGC
GCCGCTGCGCGACCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGATAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8490.65 Da
• Isoelectric Point: 8.0827

>AT226053 WP_038273164.1 NZ_CP087696:46340-46561 [Xylella taiwanensis]
MAFIHEPTSKVARHRKQMRAAGLRPVQFWVPDTRTPEFAAEIRSQCRSLKNDPEEADALRFTEEAITHIEGWK

Nucleotide

• Length: 222 bp

>AT226053 NZ_CP121766:3159925-3160479 [Pseudomonas aeruginosa]
ATGACCAAGCCTCTCCCCTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCACCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAAGCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGCTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCCCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTACGCCTGGTGGAAACCCTCTGA

Structures

Toxin

• AlphaFold DB: Z9JGT1

Antitoxin

• AlphaFold DB: Z9JGL9