Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 60067..60794 | Replicon | plasmid pDD01845-1 |
| Accession | NZ_CP087664 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain DD01845 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | LMH56_RS27040 | Protein ID | WP_011251285.1 |
| Coordinates | 60483..60794 (-) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | LMH56_RS27035 | Protein ID | WP_011251286.1 |
| Coordinates | 60067..60486 (-) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LMH56_RS27005 (LMH56_27005) | 55235..55705 | - | 471 | WP_048333570.1 | hypothetical protein | - |
| LMH56_RS27010 (LMH56_27010) | 56018..56653 | - | 636 | WP_223171879.1 | hypothetical protein | - |
| LMH56_RS27015 (LMH56_27015) | 57072..57692 | + | 621 | WP_223175004.1 | conjugative transfer protein MobI(A/C) | - |
| LMH56_RS27020 (LMH56_27020) | 57713..58501 | + | 789 | WP_040217257.1 | hypothetical protein | - |
| LMH56_RS27025 (LMH56_27025) | 58515..58880 | + | 366 | WP_048333448.1 | hypothetical protein | - |
| LMH56_RS27030 (LMH56_27030) | 58952..59920 | - | 969 | WP_074428168.1 | IS5 family transposase | - |
| LMH56_RS27035 (LMH56_27035) | 60067..60486 | - | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| LMH56_RS27040 (LMH56_27040) | 60483..60794 | - | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| LMH56_RS27045 (LMH56_27045) | 60999..61436 | - | 438 | Protein_60 | DDE-type integrase/transposase/recombinase | - |
| LMH56_RS27050 (LMH56_27050) | 61571..62268 | + | 698 | WP_223175001.1 | IS1-like element IS1A family transposase | - |
| LMH56_RS27055 (LMH56_27055) | 62270..62719 | + | 450 | WP_011251283.1 | thioredoxin fold domain-containing protein | - |
| LMH56_RS27060 (LMH56_27060) | 62736..63047 | + | 312 | WP_011251282.1 | hypothetical protein | - |
| LMH56_RS27065 (LMH56_27065) | 63061..63402 | + | 342 | WP_011251281.1 | hypothetical protein | - |
| LMH56_RS27070 (LMH56_27070) | 63462..64418 | + | 957 | WP_011251280.1 | DsbA family protein | - |
| LMH56_RS27075 (LMH56_27075) | 64843..65283 | - | 441 | WP_011251275.1 | hypothetical protein | - |
| LMH56_RS27080 (LMH56_27080) | 65289..65783 | - | 495 | WP_011251274.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 | 1..219069 | 219069 | |
| - | inside | IScluster/Tn | - | - | 43396..67703 | 24307 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T225902 WP_011251285.1 NZ_CP087664:c60794-60483 [Klebsiella pneumoniae subsp. pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
>T225902 NZ_CP121527:1251533-1251721 [Staphylococcus epidermidis]
ATGAGCTTACATTTCCAAATTTTGCTGTGGCTTTCTATCTTATTTATCATCGCAGGAACGATATTATTGGTAACAATGCT
TAAAACTAAAAAAGAAGAACGAAAAGAATCTTATCTAGGCTTTACTGTAATTTTCTTAATCTTTGGTTTTGCCATCTTAA
TTTATACTTTTATATTCGGAATTTTATAA
ATGAGCTTACATTTCCAAATTTTGCTGTGGCTTTCTATCTTATTTATCATCGCAGGAACGATATTATTGGTAACAATGCT
TAAAACTAAAAAAGAAGAACGAAAAGAATCTTATCTAGGCTTTACTGTAATTTTCTTAATCTTTGGTTTTGCCATCTTAA
TTTATACTTTTATATTCGGAATTTTATAA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT225902 WP_011251286.1 NZ_CP087664:c60486-60067 [Klebsiella pneumoniae subsp. pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
>AT225902 NZ_CP121527:1251251-1251508 [Staphylococcus epidermidis]
ATGAAATTAAACAAAGCAAATATATTCAATTTGATATTCACCATTTTATTTTTTTCATTTAACATTCTCATAACCTATAA
CGCAAATATAGATTATAAGTTATGGTTAATACCTGGTTTAGCAATTTGTGGTTTTGCTCTTTTTTCAAGCTTGACCTTAG
TAATTATCTATAGTGATTTATTCAGTGAAATATTATTTTTTATCAACATTATCTTAGCACTGTATTACATTTATCCGATT
TTTTATGAATTTGTCTAA
ATGAAATTAAACAAAGCAAATATATTCAATTTGATATTCACCATTTTATTTTTTTCATTTAACATTCTCATAACCTATAA
CGCAAATATAGATTATAAGTTATGGTTAATACCTGGTTTAGCAATTTGTGGTTTTGCTCTTTTTTCAAGCTTGACCTTAG
TAATTATCTATAGTGATTTATTCAGTGAAATATTATTTTTTATCAACATTATCTTAGCACTGTATTACATTTATCCGATT
TTTTATGAATTTGTCTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|