Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5387626..5388251 Replicon chromosome
Accession NZ_CP087651
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD01653

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LL272_RS26560 Protein ID WP_002882817.1
Coordinates 5387626..5388009 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LL272_RS26565 Protein ID WP_004150355.1
Coordinates 5388009..5388251 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LL272_RS26545 (LL272_26545) 5384992..5385894 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LL272_RS26550 (LL272_26550) 5385891..5386526 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LL272_RS26555 (LL272_26555) 5386523..5387452 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LL272_RS26560 (LL272_26560) 5387626..5388009 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LL272_RS26565 (LL272_26565) 5388009..5388251 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LL272_RS26570 (LL272_26570) 5388456..5389373 + 918 WP_004181612.1 alpha/beta hydrolase -
LL272_RS26575 (LL272_26575) 5389387..5390328 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LL272_RS26580 (LL272_26580) 5390373..5390810 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LL272_RS26585 (LL272_26585) 5390807..5391667 - 861 WP_004146232.1 virulence factor BrkB family protein -
LL272_RS26590 (LL272_26590) 5391661..5392260 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T225849 WP_002882817.1 NZ_CP087651:c5388009-5387626 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T225849 NZ_CP121476:c2907038-2906742 [Pseudomonas sp. 905_Psudmo1]
ATGAAAAAGCGCAAACCCTATTTCAAGCTTGAGCTGGTGAAGCAGGCCGTAACCGAGCAGCGTTATCGCTTCACCCGCGT
AGCCTTGGAAGGGGGCGCCGAGCTGGGTATGGAGATGGCTGACATGCTGGCCGTTATCAGTGCCCTGAGCAGCCGCGACT
TTTTCAAGAGCATGACAACCTATGCGGATCATACTACCTGGCAGGACGTTTACCTACCCGACACCGAATTCGGGCAGGTC
TATCTGAAGTTCACGCTGGTGGCCGATCTGCTGATCGTTTCCTTCAAGGAGAAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT225849 WP_004150355.1 NZ_CP087651:c5388251-5388009 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT225849 NZ_CP121476:c2906739-2906338 [Pseudomonas sp. 905_Psudmo1]
ATGAAATGTCCAGTATGCGGCGGGGCGGAACTGGTTCACGACACCCGCGATATGCCCTTCACCTACAAGGGGCAGACCAC
CCAAATTACTGCAGTAACGGCTGACTGGTGCGATGCGTGCGGCGAGTCCCTGACTGGGCCGGCTGAGAGTGAGCGCGTTA
TGCGTGCCATGAACGAGTTTCGCCAGCAGGTGAACGCCCAGGACGGCAATCAGGAGCTTATTCGCAGCGTGCGTAAGCAG
TTGCGGTTGAGCCAACGTGAAGCGGCCGAGCTGTTCGGCGGTGGGCCGAATGCGTTTTCTCGTTACGAGCGAGGCAGCAC
AGAAGCACCGCAACCGCTGGTGCAGTTGTTCAAAATCTTGGGGCGACACCCGGAGTTGATCAACGAGCTGCGTGCTGGTT
GA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References