Detailed information of TA system TA225500

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 98196..98622
• Replicon: plasmid p10_578_1
• Accession: NZ_CP087565
• Organism: Escherichia coli strain CM2010-0578/1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LOF66_RS25400
• Protein ID: WP_001372321.1
• Coordinates: 98196..98321 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	98398..98622 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOF66_RS25355 (93243)	93243..93470	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
LOF66_RS25360 (93564)	93564..94250	-	687	WP_000332484.1	PAS domain-containing protein	-
LOF66_RS25365 (94441)	94441..94824	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LOF66_RS25370 (95101)	95101..95748	+	648	WP_133301285.1	transglycosylase SLT domain-containing protein	-
LOF66_RS25375 (96045)	96045..96866	-	822	WP_001234469.1	DUF932 domain-containing protein	-
LOF66_RS25380 (96987)	96987..97274	-	288	WP_000107535.1	hypothetical protein	-
LOF66_RS25385 (97299)	97299..97505	-	207	WP_000547939.1	hypothetical protein	-
LOF66_RS25390 (97575)	97575..97748	+	174	Protein_105	hypothetical protein	-
LOF66_RS25395 (97746)	97746..97976	-	231	WP_071587244.1	hypothetical protein	-
LOF66_RS25400 (98196)	98196..98321	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LOF66_RS25405 (98263)	98263..98412	-	150	Protein_108	plasmid maintenance protein Mok	-
- (98398)	98398..98622	-	225	NuclAT_0	-	Antitoxin
- (98398)	98398..98622	-	225	NuclAT_0	-	Antitoxin
- (98398)	98398..98622	-	225	NuclAT_0	-	Antitoxin
- (98398)	98398..98622	-	225	NuclAT_0	-	Antitoxin
LOF66_RS25410 (98434)	98434..98622	+	189	WP_001299721.1	hypothetical protein	-
LOF66_RS25415 (98591)	98591..99353	-	763	Protein_110	plasmid SOS inhibition protein A	-
LOF66_RS25420 (99350)	99350..99784	-	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
LOF66_RS25425 (99839)	99839..101797	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
LOF66_RS25430 (101856)	101856..102089	-	234	WP_000006004.1	DUF905 domain-containing protein	-
LOF66_RS25435 (102145)	102145..102672	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
LOF66_RS25440 (103145)	103145..103423	-	279	WP_072127934.1	hypothetical protein	-
LOF66_RS25445 (103315)	103315..103575	-	261	WP_021536188.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN	1..128397	128397

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T225500 WP_001372321.1 NZ_CP087565:c98321-98196 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T225500 NZ_CP121154:13680-14096 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT225500 NZ_CP087565:c98622-98398 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin