Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2549094..2549893 Replicon chromosome
Accession NZ_CP087564
Organism Escherichia coli strain CM2010-0578/1

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag LOF66_RS12925 Protein ID WP_000347273.1
Coordinates 2549429..2549893 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LOF66_RS12920 Protein ID WP_001307405.1
Coordinates 2549094..2549429 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOF66_RS12905 (2544879) 2544879..2545649 - 771 WP_106108539.1 2-dehydro-3-deoxyglucarate aldolase -
LOF66_RS12910 (2545665) 2545665..2546999 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
LOF66_RS12915 (2547374) 2547374..2548945 + 1572 WP_001273753.1 galactarate dehydratase -
LOF66_RS12920 (2549094) 2549094..2549429 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LOF66_RS12925 (2549429) 2549429..2549893 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LOF66_RS12930 (2549948) 2549948..2550757 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LOF66_RS12935 (2551006) 2551006..2552286 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
LOF66_RS12940 (2552309) 2552309..2552782 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
LOF66_RS12945 (2552793) 2552793..2553572 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
LOF66_RS12950 (2553562) 2553562..2554440 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LOF66_RS12955 (2554458) 2554458..2554892 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T225491 WP_000347273.1 NZ_CP087564:2549429-2549893 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T225491 NZ_CP121153:3235990-3236376 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAAGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAGGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT225491 WP_001307405.1 NZ_CP087564:2549094-2549429 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT225491 NZ_CP121153:3236366-3236695 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References