Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1228340..1229171 Replicon chromosome
Accession NZ_CP087564
Organism Escherichia coli strain CM2010-0578/1

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag LOF66_RS06435 Protein ID WP_000854814.1
Coordinates 1228797..1229171 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag LOF66_RS06430 Protein ID WP_001285585.1
Coordinates 1228340..1228708 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOF66_RS06405 (1223804) 1223804..1224676 + 873 WP_001069814.1 GTPase family protein -
LOF66_RS06410 (1224894) 1224894..1227290 + 2397 WP_268083935.1 dynamin family protein -
LOF66_RS06415 (1227320) 1227320..1227490 + 171 Protein_1262 antirestriction protein -
LOF66_RS06420 (1227506) 1227506..1227982 + 477 WP_001186773.1 RadC family protein -
LOF66_RS06425 (1228045) 1228045..1228266 + 222 WP_000692323.1 DUF987 domain-containing protein -
LOF66_RS06430 (1228340) 1228340..1228708 + 369 WP_001285585.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
LOF66_RS06435 (1228797) 1228797..1229171 + 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
LOF66_RS06440 (1229168) 1229168..1229362 + 195 WP_000988600.1 DUF5983 family protein -
LOF66_RS06445 (1229408) 1229408..1229488 + 81 Protein_1268 hypothetical protein -
LOF66_RS06450 (1229777) 1229777..1229905 - 129 Protein_1269 transposase domain-containing protein -
LOF66_RS06455 (1230025) 1230025..1230159 + 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
LOF66_RS06460 (1230260) 1230260..1230589 - 330 WP_000450409.1 DUF496 family protein -
LOF66_RS06465 (1230761) 1230761..1231819 - 1059 WP_001200891.1 FUSC family protein -
LOF66_RS06470 (1232017) 1232017..1232490 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
LOF66_RS06475 (1232609) 1232609..1233775 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T225484 WP_000854814.1 NZ_CP087564:1228797-1229171 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T225484 NZ_CP121153:1083889-1084224 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTCAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTTGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT225484 WP_001285585.1 NZ_CP087564:1228340-1228708 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT225484 NZ_CP121153:1083641-1083889 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCTCCTGCAGTGCGGATCCCAGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGGCCTGGTGGATGGCAAATTAATTATTGAGCCAGTACGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAGCGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References