Detailed information of TA system TA225214

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 318726..319560
• Replicon: chromosome
• Accession: NZ_CP087287
• Organism: Escherichia coli strain APEC 16-1068

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: LO742_RS01470
• Protein ID: WP_000854820.1
• Coordinates: 318726..319103 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: LO742_RS01475
• Protein ID: WP_001285610.1
• Coordinates: 319192..319560 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO742_RS01450 (315289)	315289..316827	-	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-
LO742_RS01455 (317662)	317662..318222	-	561	Protein_278	DUF4942 domain-containing protein	-
LO742_RS01460 (318307)	318307..318504	-	198	WP_085949158.1	DUF957 domain-containing protein	-
LO742_RS01465 (318532)	318532..318729	-	198	Protein_280	DUF5983 family protein	-
LO742_RS01470 (318726)	318726..319103	-	378	WP_000854820.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
LO742_RS01475 (319192)	319192..319560	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LO742_RS01480 (319640)	319640..319861	-	222	WP_000692347.1	DUF987 domain-containing protein	-
LO742_RS01485 (319948)	319948..320424	-	477	WP_001186193.1	RadC family protein	-
LO742_RS01490 (320439)	320439..320918	-	480	WP_000706978.1	antirestriction protein	-
LO742_RS01495 (321018)	321018..321158	+	141	WP_000997937.1	hypothetical protein	-
LO742_RS01500 (321184)	321184..322002	-	819	WP_001175155.1	DUF932 domain-containing protein	-
LO742_RS01505 (322092)	322092..322325	-	234	WP_000883181.1	DUF905 family protein	-
LO742_RS01510 (322331)	322331..323008	-	678	WP_001097565.1	hypothetical protein	-
LO742_RS01515 (323159)	323159..323839	-	681	WP_001278649.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T225214 WP_000854820.1 NZ_CP087287:c319103-318726 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T225214 NZ_CP121074:c49046-48630 [Salmonella enterica subsp. enterica serovar Infantis]
GTGAAGAAAATGTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCCGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCACCGTATTGTGGTCTCGGCCATCACCTACTCAGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGTCACATTGAACTGGTTGACGCGTTCTGCGCCCGCCTCGATGCTATCCTGCCCTGGGACCGGGCC
GCGGTGAACGCCACCACGGAGATTAAGGTGGCGCTACGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCGATCGC
CGGGCACGCTATTGCCGCCGGCGCGGTACTGGTGACAAATAATACGCGGGAGTTTGAGCGGGTGCCGGGTCTTGTTCTGG
AAGACTGGGGAAGATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT225214 WP_001285610.1 NZ_CP087287:c319560-319192 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT225214 NZ_CP121074:c49273-49043 [Salmonella enterica subsp. enterica serovar Infantis]
ATGAGAACCGTATCTATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGATGGAGTGAG
CGAGCTGGAAATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCTGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTCG
AAAAAGCTGACCCGGGCTTTATGGCGGAGCGTGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6