Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 757443..758039 Replicon chromosome
Accession NZ_CP087198
Organism Pseudomonas sp. B21-010

Toxin (Protein)


Gene name PumA Uniprot ID A0A2Z4ZU46
Locus tag LOY50_RS03295 Protein ID WP_024617343.1
Coordinates 757737..758039 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A2K4FWQ4
Locus tag LOY50_RS03290 Protein ID WP_024617344.1
Coordinates 757443..757733 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOY50_RS03270 (LOY50_03260) 753641..755767 - 2127 WP_258690283.1 TonB-dependent copper receptor -
LOY50_RS03275 (LOY50_03265) 755850..756239 - 390 WP_253478390.1 DUF2946 domain-containing protein -
LOY50_RS03280 (LOY50_03270) 756297..756776 - 480 WP_018611909.1 copper chaperone PCu(A)C -
LOY50_RS03285 (LOY50_03275) 756824..757222 - 399 WP_253471333.1 DUF2946 domain-containing protein -
LOY50_RS03290 (LOY50_03280) 757443..757733 - 291 WP_024617344.1 putative addiction module antidote protein Antitoxin
LOY50_RS03295 (LOY50_03285) 757737..758039 - 303 WP_024617343.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LOY50_RS03300 (LOY50_03290) 758232..758960 - 729 WP_258690284.1 cobalt-precorrin-6A reductase -
LOY50_RS03305 (LOY50_03295) 758957..760054 - 1098 WP_258690285.1 cobalt-precorrin-5B (C(1))-methyltransferase -
LOY50_RS03310 (LOY50_03300) 760047..761258 - 1212 WP_253471337.1 precorrin-6y C5,15-methyltransferase (decarboxylating) subunit CbiE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-96)

Antitoxin

Uncharacterized protein HI1420

(4-95)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11181.72 Da        Isoelectric Point: 8.9343

>T225109 WP_024617343.1 NZ_CP087198:c758039-757737 [Pseudomonas sp. B21-010]
MIEFEQSRVFSSWLNGLKDIAGKARIIARLRSAEHGNFGDCEPVGNAVYEMRIHSGPGYRVYFSRQGSVLYLLLVGGDKS
TQKRDIARAVQMAQTMGNEE

Download         Length: 303 bp

>T225109 NZ_CP120971:c91932-91582 [Moellerella wisconsensis]
ATGTGGGAAATATTAACGAGAGATCTCTTTGATTCTTGGTTCGAAGAGCAAAATGAGGAAACTCAGATTGAAGTGTTGGC
AGTGTTAATGATTTTACGTGAGGATGGCCCCAATCTTGGACGCCCTCAAGTTGACACCCTGAAGGGCTCACAGTTTCCCA
ACATGAAGGAACTGCGAATTCAGGTTGGTGGTCATCCGATTAGAGCGTGTTTCGCATTTGACCCAATTCGTCGTGGCATT
GTGTTATGTGCCGGTGATAAGAAAGGAAAAGATGAAACCCGTTTCTACAAGAAATTAATCAAGATGGCCGATGCGGAATA
TGCAGCGCACTTATTAGAGCAGGAGAAATAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10403.92 Da        Isoelectric Point: 4.8096

>AT225109 WP_024617344.1 NZ_CP087198:c757733-757443 [Pseudomonas sp. B21-010]
MTEEFTRFDAATYLKTPEEMAAYLDACFEEDAGDGVLVRAALNDIARAKGMTQIARDAGLGRESLYKALGSTGNPEFTTI
MKVMKALGLRLHATAI

Download         Length: 291 bp

>AT225109 NZ_CP120971:c91580-91281 [Moellerella wisconsensis]
ATGTCCACTTTAAATGAACTACTTGCAAAACAATCCCCCGAAACGCAGGCAAAAATTGAAGACCGAGCAGAAGAAATCCG
GCGTGAAATCACACTAGCTAAAATTCGTGAGGAACTGAAACTTTCTCAAACTGAGCTAGCTCAATCACTGGGCGTTTCGC
AACCTTCTATTGCTAAGCTTGAAAATGTTGATAACGATCCGAAGCTTTCAACTCTGAAGCGCTACATTAAAGCTTTAGGC
GGTGAACTTTCTATCGATGTAACGTTACCGAATGGTAAGAGAATTGGATTGCATCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z4ZU46

Antitoxin

Source ID Structure
AlphaFold DB A0A2K4FWQ4

References