Detailed information of TA system TA224988

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09812-MazE
• Location: 467221..467852
• Replicon: chromosome
• Accession: NZ_CP087171
• Organism: Pseudomonas atacamensis strain B21-045

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LOY27_RS02065
• Protein ID: WP_258710889.1
• Coordinates: 467490..467852 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: J2YH56
• Locus tag: LOY27_RS02060
• Protein ID: WP_008077592.1
• Coordinates: 467221..467490 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY27_RS02025 (LOY27_02020)	462719..463264	-	546	WP_016772027.1	membrane protein	-
LOY27_RS02030 (LOY27_02025)	463261..463515	-	255	WP_016772026.1	acyl carrier protein	-
LOY27_RS02035 (LOY27_02030)	463525..463800	-	276	WP_122699832.1	phosphopantetheine-binding protein	-
LOY27_RS02040 (LOY27_02035)	463778..464590	-	813	WP_016772024.1	lysophospholipid acyltransferase family protein	-
LOY27_RS02045 (LOY27_02040)	464566..465291	-	726	WP_016772023.1	beta-ketoacyl synthase chain length factor	-
LOY27_RS02050 (LOY27_02045)	465648..466418	+	771	WP_016772022.1	ParA family protein	-
LOY27_RS02055 (LOY27_02050)	466550..466984	-	435	WP_258710888.1	thioredoxin TrxC	-
LOY27_RS02060 (LOY27_02055)	467221..467490	+	270	WP_008077592.1	hypothetical protein	Antitoxin
LOY27_RS02065 (LOY27_02060)	467490..467852	+	363	WP_258710889.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LOY27_RS02070 (LOY27_02065)	467858..469321	-	1464	WP_258710890.1	YdiU family protein	-
LOY27_RS02075 (LOY27_02070)	469370..472723	-	3354	WP_258710891.1	mechanosensitive channel MscK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13409.30 Da
• Isoelectric Point: 6.4635

>T224988 WP_258710889.1 NZ_CP087171:467490-467852 [Pseudomonas atacamensis]
MVRRQPPQRGDVYWIDPNPVAGREMMNRHRFVVITPREINALGVSMTVPVTSGENFSRNMGLAVAITGHETNGVAVCNQV
RSFDIEQRVRDGTAKFIERLDDVTMSDIVDRVISAIDPLS

Nucleotide

• Length: 363 bp

>T224988 NZ_CP120687:c809065-808847 [Lacticaseibacillus sp. DSM 115425]
ATCAGTTGGTTTGAGCAAGCTGATAAAGCCACGCTCAGAAAAATTAATCGGCTCATCACATCGATCGACCGGACACCGTT
TGATAGCCTGGGTAAACCAGAACCATTGAAACACGGCTTGTCTGGTATGTGGTCGCGCCGAATCACGCAAGAACACCGCT
TAATTTACGAAATTGAGGCAAACACGATGACAATTATTTCCTGTCGCGATCATTACTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9521.94 Da
• Isoelectric Point: 5.1069

>AT224988 WP_008077592.1 NZ_CP087171:467221-467490 [Pseudomonas atacamensis]
MSVTSKIRRQGGAAVITIPPTLLKLLHLEVGAQLELNVVDGELIARPAVAATRKRYSLSELLEGAEELQKLNAQTSETLE
GDSVGRELG

Nucleotide

• Length: 270 bp

>AT224988 NZ_CP120687:c809372-809097 [Lacticaseibacillus sp. DSM 115425]
ATGGACGCTGTTTCCTACTCAGTTTTCCGCCGCAATCTCAAGCACTACCTTCAAGCAGTCAATGACACCTCCACGCCACT
CTTGATTACTAGCCGTGATAATGAGGCTGATACTGCCGTCTTGATGAATAAACGTGATTTTGATGCCATGCAAGAAACGA
TGAGGATTTTGTCGAATCCTGAACTCATGGCCAAGATCCGCCGTGGTCAAGCCCAAATTGCCGCTGGTAAAACCCGGCAA
CACGATTTAAAGGATGTCGGTGGCGGGGATGATTAA

Structures

Antitoxin

• AlphaFold DB: J2YH56