Detailed information of TA system TA022495

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ygfYX/Cpta(toxin)
• Location: 1251978..1252614
• Replicon: chromosome
• Accession: NC_009901
• Organism: Shewanella pealeana ATCC 700345

Toxin (Protein)

• Gene name: ygfX
• Uniprot ID: A8H1B9
• Locus tag: SPEA_RS05370
• Protein ID: WP_012154287.1
• Coordinates: 1252198..1252614 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: ygfY
• Uniprot ID: A8H1B8
• Locus tag: SPEA_RS05365
• Protein ID: WP_012154286.1
• Coordinates: 1251978..1252217 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPEA_RS05340	1247057..1247857	+	801	WP_012154281.1	sulfite exporter TauE/SafE family protein	-
SPEA_RS05345	1247941..1248792	+	852	WP_012154282.1	thymidylate synthase	-
SPEA_RS05350	1249284..1250459	+	1176	WP_012154283.1	Na+/H+ antiporter NhaA	-
SPEA_RS05355	1250456..1250839	+	384	WP_012154284.1	hypothetical protein	-
SPEA_RS05360	1250909..1251817	+	909	WP_012154285.1	transcriptional activator NhaR	-
SPEA_RS05365	1251978..1252217	+	240	WP_012154286.1	succinate dehydrogenase assembly factor 2	Antitoxin
SPEA_RS05370	1252198..1252614	+	417	WP_012154287.1	hypothetical protein	Toxin
SPEA_RS05375	1252672..1254282	-	1611	WP_012154288.1	L-aspartate oxidase	-
SPEA_RS05380	1254502..1255080	+	579	WP_012154289.1	RNA polymerase sigma factor RpoE	-
SPEA_RS05385	1255104..1255709	+	606	WP_012154290.1	anti-sigma factor	-
SPEA_RS05390	1255722..1256654	+	933	WP_012154291.1	MucB/RseB C-terminal domain-containing protein	-
SPEA_RS05395	1256668..1257132	+	465	WP_012154292.1	SoxR reducing system RseC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (5-134)

Antitoxin

• Flavinator of succinate dehydrogenase (5-74)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15978.82 Da
• Isoelectric Point: 9.2383

>T22495 WP_012154287.1 NC_009901:1252198-1252614 [Shewanella pealeana ATCC 700345]
VDVQQHSFDLISSNGQRLSLTVIALICLSSFLIWPYAENTVYILLKFLLFSACCTFFIWQIYRLKHWRCQFVLKADGGGR
FKYGTDFVLQGKPVITPFAVMFDVTFAGGKKRLVLWADMLDDTNYRHLCRLLQLASQR

Nucleotide

• Length: 417 bp

>T22495 NC_009901:1252198-1252614 [Shewanella pealeana ATCC 700345]
GTGGACGTCCAGCAGCATAGCTTCGATCTGATTTCTTCCAACGGTCAGCGACTCTCTCTGACCGTTATTGCCCTTATCTG
TTTAAGCTCTTTCCTTATTTGGCCTTACGCTGAAAATACCGTTTATATCTTGCTCAAATTTCTATTATTCAGTGCCTGCT
GTACCTTTTTTATTTGGCAGATTTATCGATTAAAGCACTGGCGATGCCAGTTTGTACTTAAGGCAGATGGAGGAGGCCGA
TTTAAATATGGGACCGACTTTGTTTTACAGGGGAAGCCTGTCATTACACCATTTGCGGTGATGTTTGATGTCACTTTTGC
GGGAGGAAAGAAAAGACTCGTACTATGGGCGGATATGTTAGACGACACAAACTATCGACATCTGTGTCGTCTATTGCAGT
TAGCTAGTCAGAGGTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9303.95 Da
• Isoelectric Point: 5.8240

>AT22495 WP_012154286.1 NC_009901:1251978-1252217 [Shewanella pealeana ATCC 700345]
MKLPRVKWACRRGMLELDVLFQPFVEQQYEAMSIKDKDTFVRLLECEDPELFAWFMGHEQCPNPAFAEMIIKVRGRPAA

Nucleotide

• Length: 240 bp

>AT22495 NC_009901:1251978-1252217 [Shewanella pealeana ATCC 700345]
ATGAAATTACCTAGAGTTAAATGGGCTTGCCGCAGAGGCATGCTGGAGCTAGATGTACTATTTCAGCCTTTTGTTGAGCA
GCAATATGAAGCGATGTCGATAAAAGATAAAGACACTTTTGTGAGATTACTCGAATGCGAAGATCCAGAATTGTTTGCTT
GGTTTATGGGACATGAGCAGTGCCCAAATCCTGCGTTTGCCGAAATGATAATCAAGGTTCGTGGACGTCCAGCAGCATAG

Structures

Toxin

• AlphaFold DB: A8H1B9

Antitoxin

• AlphaFold DB: A8H1B8