Detailed information of TA system TA224948

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-DUF971
• Location: 8223..8821
• Replicon: plasmid unnamed2
• Accession: NZ_CP087162
• Organism: Pseudomonas sp. CIP-10

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A6H1Q9F7
• Locus tag: LMH93_RS29260
• Protein ID: WP_028692294.1
• Coordinates: 8519..8821 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: LMH93_RS29255
• Protein ID: WP_028692295.1
• Coordinates: 8223..8522 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH93_RS29235 (LMH93_29235)	3594..4877	-	1284	WP_028691924.1	Y-family DNA polymerase	-
LMH93_RS29240 (LMH93_29240)	4867..5295	-	429	WP_028691923.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
LMH93_RS29245 (LMH93_29245)	7364..7687	-	324	WP_028692297.1	hypothetical protein	-
LMH93_RS29250 (LMH93_29250)	7982..8203	-	222	WP_028692296.1	hypothetical protein	-
LMH93_RS29255 (LMH93_29255)	8223..8522	-	300	WP_028692295.1	putative addiction module antidote protein	Antitoxin
LMH93_RS29260 (LMH93_29260)	8519..8821	-	303	WP_028692294.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LMH93_RS29265 (LMH93_29265)	9024..9803	+	780	WP_028692293.1	DUF4942 domain-containing protein	-
LMH93_RS29270 (LMH93_29270)	9956..10324	+	369	WP_028692292.1	hypothetical protein	-
LMH93_RS29275 (LMH93_29275)	10324..10668	+	345	WP_226342977.1	theronine dehydrogenase	-
LMH93_RS29280 (LMH93_29280)	11006..12097	+	1092	WP_051555741.1	hypothetical protein	-
LMH93_RS29285 (LMH93_29285)	12243..12485	+	243	WP_028692289.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aac(6')-Ib-cr / qnrVC1 / blaOXA-10 / ant(3'')-Ia / dfrA14	-	1..61278	61278

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Uncharacterized protein HI1420 (7-94)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11277.85 Da
• Isoelectric Point: 10.3903

>T224948 WP_028692294.1 NZ_CP087162:c8821-8519 [Pseudomonas sp. CIP-10]
MKTIKQTATYRNWERKLRDKQAKAIIAARVFRVAHGLLGDVQPVGQGISELRIHHGPGYRVYFQQRGNQLVLLLCGGDKS
SQARDIETAKSLASQWSDDE

Nucleotide

• Length: 303 bp

>T224948 NZ_CP120637:c1242122-1241376 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTGAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10825.27 Da
• Isoelectric Point: 5.1084

>AT224948 WP_028692295.1 NZ_CP087162:c8522-8223 [Pseudomonas sp. CIP-10]
MKEQFYDYDPADALTDAESIAVFMEDAFETGDAAYIAKCLGVVARAKGMTALANESGLSREHLYRSFSDKGNPTLKTLLA
VMRAVGLDLTTRKHVEPAH

Nucleotide

• Length: 300 bp

>AT224948 NZ_CP120637:c1241375-1241205 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: A0A6H1Q9F7