Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4195428..4196047 Replicon chromosome
Accession NZ_CP087146
Organism Klebsiella pneumoniae strain KP16

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LOX92_RS20905 Protein ID WP_002892050.1
Coordinates 4195829..4196047 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LOX92_RS20900 Protein ID WP_002892066.1
Coordinates 4195428..4195802 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOX92_RS20890 (LOX92_20890) 4190580..4191773 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LOX92_RS20895 (LOX92_20895) 4191796..4194942 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
LOX92_RS20900 (LOX92_20900) 4195428..4195802 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LOX92_RS20905 (LOX92_20905) 4195829..4196047 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LOX92_RS20910 (LOX92_20910) 4196206..4196772 + 567 WP_002892030.1 maltose O-acetyltransferase -
LOX92_RS20915 (LOX92_20915) 4196744..4196884 - 141 WP_004147370.1 hypothetical protein -
LOX92_RS20920 (LOX92_20920) 4196905..4197375 + 471 WP_002892026.1 YlaC family protein -
LOX92_RS20925 (LOX92_20925) 4197350..4198801 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
LOX92_RS20930 (LOX92_20930) 4198902..4199600 + 699 WP_002892021.1 GNAT family protein -
LOX92_RS20935 (LOX92_20935) 4199597..4199737 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LOX92_RS20940 (LOX92_20940) 4199737..4200000 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T224902 WP_002892050.1 NZ_CP087146:4195829-4196047 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T224902 NZ_CP120609:2600360-2600710 [Priestia megaterium DSM 319]
TTGGTAGTCAAACGTGGCGACGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGAGGCGTTCGCCCTGT
ACTTGTCATTCAAAATGATATAGGCAATCGTTTTAGCCCAACTGTTATTGTAGCTGCGATCACTGCTCAAATTCAAAAGG
CAAAGTTACCTACGCATGTTGAAATTGATGCAAAGCGCTATGGATTTGAGCGAGATTCAGTTATTTTACTAGAACAAATT
CGTACTATTGATAAACAGAGGCTAACAGACAAGATTACCCATTTAGATGATGAAATGATGGACAGAGTGGATGAAGCCTT
GCAAATCAGTGTAGGACTTATCGACTTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT224902 WP_002892066.1 NZ_CP087146:4195428-4195802 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT224902 NZ_CP120609:2600074-2600355 [Priestia megaterium DSM 319]
GTGTCTGAAGCGAGCGCAACTACAGAAATCCTAGTAAGATTACCGAAAAATTTAGTATCGGAGCTCGATCTTATGGTTAA
ACAAGAAAATGGCAACCGAAGTGATTTTATTCACCAAGCAACAAAATTGTACCTTCGTGAGCGAAAAAAACGCCATATTC
GCGAATCAATGAGACGTGGATATATGGAGATGGCAAAGATAAACTTAAATATTGCTTCCGAGGCTTTTCTAGCAGAGTAT
GAAGCTGATCATACTGTGGATCGTTTAGTAAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References