Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 4903537..4905160 Replicon chromosome
Accession NZ_CP087110
Organism Escherichia coli strain PJ-T13

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag LM520_RS23930 Protein ID WP_000088046.1
Coordinates 4903835..4905160 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag LM520_RS23925 Protein ID WP_024136327.1
Coordinates 4903537..4903830 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LM520_RS23900 (4899409) 4899409..4900650 + 1242 WP_001053338.1 VWA domain-containing protein -
LM520_RS24180 (4900741) 4900741..4900872 + 132 WP_000374059.1 hypothetical protein -
LM520_RS23905 (4901213) 4901213..4901494 - 282 WP_000077926.1 hypothetical protein -
LM520_RS23910 (4901544) 4901544..4901735 - 192 WP_000398480.1 hypothetical protein -
LM520_RS23915 (4901827) 4901827..4902168 - 342 WP_001151575.1 hypothetical protein -
LM520_RS23920 (4902541) 4902541..4902933 + 393 WP_000341066.1 hypothetical protein -
LM520_RS23925 (4903537) 4903537..4903830 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
LM520_RS23930 (4903835) 4903835..4905160 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
LM520_RS23935 (4905221) 4905221..4905427 + 207 WP_000134171.1 hypothetical protein -
LM520_RS23940 (4905529) 4905529..4905939 + 411 WP_000985911.1 hypothetical protein -
LM520_RS23945 (4905952) 4905952..4906767 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
LM520_RS23950 (4907021) 4907021..4907446 + 426 WP_001043843.1 hypothetical protein -
LM520_RS23955 (4907995) 4907995..4908303 + 309 WP_001224687.1 hypothetical protein -
LM520_RS23960 (4908319) 4908319..4909176 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
LM520_RS23965 (4909238) 4909238..4909441 + 204 WP_001194555.1 hypothetical protein -
LM520_RS23970 (4909783) 4909783..4910139 - 357 WP_065311124.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element aph(6)-Id / tet(A) / sul3 / aac(3)-IId / blaLAP-2 / blaTEM-1B / blaCTX-M-55 / qnrS1 / mph(A) / ARR-3 / dfrA14 / floR - 4632782..4927985 295203


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T224753 WP_000088046.1 NZ_CP087110:4903835-4905160 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T224753 NZ_CP120424:2099704-2099892 [Staphylococcus epidermidis]
ATGAGCTTACATTTCCAAATTTTGCTGTGGCTTTCTATCTTATTTATCATCGCAGGAACGATATTATTGGTAACAATGCT
TAAAACTAAAAAAGAAGAACGAAAAGAATCTTATCTAGGCTTTACTGTAATTTTCTTAATCTTTGGTTTTGCCATCTTAA
TTTATACTTTTATATTCGGAATTTTATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT224753 WP_024136327.1 NZ_CP087110:4903537-4903830 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT224753 NZ_CP120424:2099422-2099679 [Staphylococcus epidermidis]
ATGAAATTAAACAAAGCAAATATATTCAATTTGATATTCACCATTTTATTTTTTTCATTTAACATTCTCATAACCTATAA
CGCAAATATAGATTATAAGTTATGGTTAATACCTGGTTTAGCAATTTGTGGTTTTGCTCTTTTTTCAAGCTTGACCTTAG
TAATTATCTATAGTGATTTATTCAGTGAAATATTATTTTTTATCAACATTATCTTAGCACTGTATTACATTTATCCGATT
TTTTATGAATTTGTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References