Detailed information of TA system TA022475

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 14815..15085
• Replicon: plasmid pAPEC-O1-ColBM
• Accession: NC_009837
• Organism: Escherichia coli APEC O1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: APECO1_RS25795
• Protein ID: WP_001312861.1
• Coordinates: 14927..15085 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	14815..14878 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APECO1_RS30710	9870..10136	+	267	WP_072254135.1	hypothetical protein	-
APECO1_RS25765	10609..11136	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
APECO1_RS25770	11192..11425	+	234	WP_000006004.1	DUF905 domain-containing protein	-
APECO1_RS25775	11484..13442	+	1959	WP_001145470.1	ParB/RepB/Spo0J family partition protein	-
APECO1_RS25780	13497..13931	+	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
APECO1_RS25785	13928..14647	+	720	WP_001276228.1	plasmid SOS inhibition protein A	-
APECO1_RS31660	14659..14847	-	189	WP_001299721.1	hypothetical protein	-
-	14659..14883	+	225	NuclAT_0	-	-
-	14659..14883	+	225	NuclAT_0	-	-
-	14659..14883	+	225	NuclAT_0	-	-
-	14659..14883	+	225	NuclAT_0	-	-
-	14815..14878	-	64	-	-	Antitoxin
APECO1_RS25795	14927..15085	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
APECO1_RS30720	15776..15982	+	207	WP_000547939.1	hypothetical protein	-
APECO1_RS25805	16007..16294	+	288	WP_000107535.1	hypothetical protein	-
APECO1_RS25810	16415..17236	+	822	WP_001234469.1	DUF945 domain-containing protein	-
APECO1_RS25815	17533..18135	-	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
APECO1_RS25820	18456..18839	+	384	WP_001151524.1	relaxosome protein TraM	-
APECO1_RS25825	19026..19715	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..174241	174241

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T22475 WP_001312861.1 NC_009837:14927-15085 [Escherichia coli APEC O1]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T22475 NC_009837:14927-15085 [Escherichia coli APEC O1]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT22475 NC_009837:c14878-14815 [Escherichia coli APEC O1]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin