Detailed information of TA system TA224706

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 171256..171797
• Replicon: plasmid P1
• Accession: NZ_CP087084
• Organism: Pseudosulfitobacter pseudonitzschiae strain MC36-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A073IW87
• Locus tag: LOE33_RS20075
• Protein ID: WP_037931244.1
• Coordinates: 171471..171797 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A073IWP4
• Locus tag: LOE33_RS20070
• Protein ID: WP_037931247.1
• Coordinates: 171256..171474 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE33_RS20050 (LOE33_20020)	166513..167520	+	1008	WP_081855484.1	PaaX family transcriptional regulator C-terminal domain-containing protein	-
LOE33_RS20055 (LOE33_20025)	167755..169302	+	1548	WP_073194592.1	AMP-binding protein	-
LOE33_RS20060 (LOE33_20030)	169357..169821	+	465	WP_051694690.1	MaoC/PaaZ C-terminal domain-containing protein	-
LOE33_RS20065 (LOE33_20035)	169825..170958	+	1134	WP_037931249.1	alpha/beta fold hydrolase	-
LOE33_RS20070 (LOE33_20040)	171256..171474	+	219	WP_037931247.1	antitoxin MazE family protein	Antitoxin
LOE33_RS20075 (LOE33_20045)	171471..171797	+	327	WP_037931244.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LOE33_RS20080 (LOE33_20050)	171971..172327	-	357	Protein_164	ATP-binding protein	-
LOE33_RS20085 (LOE33_20055)	172317..173889	-	1573	Protein_165	IS21 family transposase	-
LOE33_RS20090 (LOE33_20060)	174225..174548	-	324	WP_027238707.1	helix-turn-helix domain-containing protein	-
LOE33_RS20095 (LOE33_20065)	174711..175118	+	408	WP_157802081.1	DoxX family protein	-
LOE33_RS20100 (LOE33_20070)	175182..175997	+	816	WP_224556226.1	class III extradiol ring-cleavage dioxygenase	-
LOE33_RS20105 (LOE33_20075)	176006..176503	+	498	WP_037931962.1	DUF5519 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..290593	290593
	-	flank	IS/Tn	-	-	172317..173603	1286

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (7-71)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11610.50 Da
• Isoelectric Point: 8.8545

>T224706 WP_037931244.1 NZ_CP087084:171471-171797 [Pseudosulfitobacter pseudonitzschiae]
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG

Nucleotide

• Length: 327 bp

>T224706 NZ_CP120396:c4597421-4597047 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGTCAAAGGGTCTGCGCTTTTTGATACCAATATCCTCATCGACCTGTTTAGCGGTCGAATTGAAGCGAAACATGCGCT
GGAAGCGTATCCGCCACAAAATGCGATTAGCCTTATCACATGGATGGAAGTGATGGTGGGTGCTAAGAAATATCATCAGG
AAAACCGCACGCGCATTGCATTAAGTGCGTTCAATATTATTGGCGTAACACAGGAAATCGCGGAACGAAGCGTCATTGTC
AGGCAGGAGTATGGCATGAAACTCCCGGATGCGATCATTCTGGCCACTGCACAAGTCCATCGTTGTGAGCTTGTGACGCG
CAATACAAAAGACTTTGCCGATATTCCGGGAGTGATCACGCCGTATCACTTGTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8175.17 Da
• Isoelectric Point: 5.8017

>AT224706 WP_037931247.1 NZ_CP087084:171256-171474 [Pseudosulfitobacter pseudonitzschiae]
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE

Nucleotide

• Length: 219 bp

>AT224706 NZ_CP120396:c4597663-4597421 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGATGTGATTAAACGGCTTGACGATCTCAAGGT
GCAACGCAACCTACCTCGTGCAGAACTCTTGCGGGAGGCCGTTGAGCAGTATCTGGAAAGACAGGATCGGGCTGAGACCA
CAATTTCGAAAGCATTGGGGCTCTGGCAAGGCTGTGAGGAAGATGGCGTTGAATACCAACGTAAACTTCGTGAGGAATGG
TAA

Structures

Toxin

• AlphaFold DB: A0A073IW87

Antitoxin

• AlphaFold DB: A0A073IWP4