Detailed information of TA system TA022467

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4404702..4405304
• Replicon: chromosome
• Accession: NC_009801
• Organism: Escherichia coli E24377A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: ECE24377A_RS23540
• Protein ID: WP_000897305.1
• Coordinates: 4404702..4405013 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECE24377A_RS23545
• Protein ID: WP_000356397.1
• Coordinates: 4405014..4405304 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECE24377A_RS23510	4399732..4400517	+	786	WP_000059674.1	DeoR/GlpR family DNA-binding transcription regulator	-
ECE24377A_RS23515	4400616..4401215	+	600	WP_001295269.1	glucose-1-phosphatase	-
ECE24377A_RS23520	4401209..4402081	+	873	WP_000920762.1	virulence factor BrkB family protein	-
ECE24377A_RS23525	4402078..4402515	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ECE24377A_RS23530	4402560..4403501	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
ECE24377A_RS23535	4403565..4404473	-	909	WP_001162704.1	alpha/beta hydrolase	-
ECE24377A_RS23540	4404702..4405013	+	312	WP_000897305.1	hypothetical protein	Toxin
ECE24377A_RS23545	4405014..4405304	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
ECE24377A_RS23550	4405909..4406127	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
ECE24377A_RS23555	4406346..4406588	+	243	WP_001086388.1	hypothetical protein	-
ECE24377A_RS23565	4406817..4407797	-	981	WP_000399648.1	IS110-like element IS621 family transposase	-
ECE24377A_RS23570	4408196..4409125	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
ECE24377A_RS23575	4409122..4409757	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4406817..4407797	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T22467 WP_000897305.1 NC_009801:4404702-4405013 [Escherichia coli O139:H28 str. E24377A]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T22467 NC_009801:4404702-4405013 [Escherichia coli O139:H28 str. E24377A]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT22467 WP_000356397.1 NC_009801:4405014-4405304 [Escherichia coli O139:H28 str. E24377A]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT22467 NC_009801:4405014-4405304 [Escherichia coli O139:H28 str. E24377A]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7