Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3481983..3482480 Replicon chromosome
Accession NZ_CP086662
Organism Escherichia coli strain E2

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LOE88_RS16640 Protein ID WP_001173343.1
Coordinates 3482307..3482480 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LOE88_RS16635 Protein ID WP_000398619.1
Coordinates 3481983..3482279 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOE88_RS16615 (3478714) 3478714..3479433 - 720 WP_000611288.1 two-component system response regulator DcuR -
LOE88_RS16620 (3479430) 3479430..3481061 - 1632 WP_001216460.1 sensor histidine kinase -
LOE88_RS16625 (3481242) 3481242..3481472 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
LOE88_RS16630 (3481484) 3481484..3481756 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
LOE88_RS16635 (3481983) 3481983..3482279 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LOE88_RS16640 (3482307) 3482307..3482480 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LOE88_RS16645 (3482599) 3482599..3484116 - 1518 WP_023155344.1 lysine--tRNA ligase -
LOE88_RS16650 (3484353) 3484353..3485810 - 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T224311 WP_001173343.1 NZ_CP086662:3482307-3482480 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T224311 NZ_CP119984:443574-443858 [Salmonella enterica]
ATGAATTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT224311 WP_000398619.1 NZ_CP086662:3481983-3482279 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT224311 NZ_CP119984:443342-443584 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCCTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGAATTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References