Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3922538..3923156 Replicon chromosome
Accession NZ_CP086626
Organism Escherichia coli strain RIVM_C037134

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LN362_RS19190 Protein ID WP_001291435.1
Coordinates 3922938..3923156 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LN362_RS19185 Protein ID WP_000344800.1
Coordinates 3922538..3922912 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN362_RS19175 (3917627) 3917627..3918820 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LN362_RS19180 (3918843) 3918843..3921992 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
LN362_RS19185 (3922538) 3922538..3922912 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LN362_RS19190 (3922938) 3922938..3923156 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LN362_RS19195 (3923328) 3923328..3923879 + 552 WP_000102564.1 maltose O-acetyltransferase -
LN362_RS19200 (3923995) 3923995..3924465 + 471 WP_000136192.1 YlaC family protein -
LN362_RS19205 (3924629) 3924629..3926179 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LN362_RS19210 (3926221) 3926221..3926574 - 354 WP_085334692.1 DUF1428 family protein -
LN362_RS19220 (3926953) 3926953..3927264 + 312 WP_000409911.1 MGMT family protein -
LN362_RS19225 (3927295) 3927295..3927867 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T224244 WP_001291435.1 NZ_CP086626:3922938-3923156 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T224244 NZ_CP119773:c2897674-2897138 [Dickeya fangzhongdai]
ATGGTAGATACTCAAAGTGAAAGTAATGGTGTAGTGGTATACGCGTATCAGGCAGATACCACATACCCTGGGAGTAAGAG
TTTCGATTGCGGAAATACCGTAATCAACTCTTTTGTGCGTAGCTCCCTCAAGAAAAGCGTCAGAGATGGGAATTGTGCAG
CTAAAGTATTGGTTAAAGATGAAACAAAAGAGCTGTTGGGTTTCTGTACTTTTGCTGCTTACTCTCTTGAAAAAAGTAAG
TTGGCGGGGGTGGTTTCAGGTTCATTGCCTCATGAATTAGGGGTTGTTCGGCTAATTATGCTGGGGGTAGCCACGAAAGA
ACAGAATAAAGGCTATGGACAAGAACTGCTTTTAGAATTCTTTAAACAGGTTAAAACCATCCACGAATCGCTACCTGTTA
AAGGCGTGTATTTGGATGCGGATCCGGCAGCAATTGACTTCTATATCCGGTTAGGTTTCGTTCAACTTAATGAACCACCG
AATGCTTTTGGTGCGGTGCCCATGTTTTTGGCCATTCAACATATCCTTGCTGCGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT224244 WP_000344800.1 NZ_CP086626:3922538-3922912 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT224244 NZ_CP119773:c2897966-2897655 [Dickeya fangzhongdai]
ATGTCTAATGTATCAGTGTTTCCCAATGATACTCGCGTCACGAAGAACGCGCGGGTGGAGTTAAAAACTAGCCCTGACCT
TAAAGATGTGCTTCGTGAAGCAGCTACGGCTGCAGGTTTAGATTTGAGTGCGTTTATTCTTAATGCCGCCCTGGAGCGAG
CAGAAAGCGTCCTGGATAACCAGCGGCGCCGACAGCTTTCAGCAGCAAGCTGGCAACAATTAAACCAACTCTTGTCTGAA
CCTGCTGCACCGACGCTGGCTTTACAGGCGTTAATGCGGAGGAAGAAACAAGATGGTAGATACTCAAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References