Detailed information of TA system TA224243

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3469487..3470192
• Replicon: chromosome
• Accession: NZ_CP086626
• Organism: Escherichia coli strain RIVM_C037134

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: LN362_RS17075
• Protein ID: WP_000539521.1
• Coordinates: 3469487..3469873 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LN362_RS17080
• Protein ID: WP_001280945.1
• Coordinates: 3469863..3470192 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN362_RS17055 (3465491)	3465491..3466117	+	627	WP_001295292.1	glutathione S-transferase GstB	-
LN362_RS17060 (3466114)	3466114..3467229	-	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
LN362_RS17065 (3467340)	3467340..3467723	-	384	WP_000497137.1	biofilm formation regulator BssR	-
LN362_RS17070 (3467936)	3467936..3469261	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
LN362_RS17075 (3469487)	3469487..3469873	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LN362_RS17080 (3469863)	3469863..3470192	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
LN362_RS17085 (3470262)	3470262..3471590	-	1329	WP_001550887.1	GGDEF domain-containing protein	-
LN362_RS17090 (3471598)	3471598..3473946	-	2349	WP_001550886.1	EAL domain-containing protein	-
LN362_RS17095 (3474124)	3474124..3475035	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T224243 WP_000539521.1 NZ_CP086626:3469487-3469873 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T224243 NZ_CP119773:c2834920-2834717 [Dickeya fangzhongdai]
ATGAAAAAAATCGACTATTTAATGCGTTTGCGTAAATGCACAACCATCGACACGCTTGAGCGTGTTATAGAAAAAAACAA
GTACGAGCTTTCCAACGATGAGCTTGAAATGTTCTATTCAGCCGCAGACCACCGCCTGGCAGAGCTGACGATGAATAAGC
TTTACGACAAAGTACCAACTGCTGTGTGGAAATACGTTCGTTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT224243 WP_001280945.1 NZ_CP086626:3469863-3470192 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT224243 NZ_CP119773:c2835342-2834974 [Dickeya fangzhongdai]
ATGGATGAGTACACACCTCAACATTATGATATTGCCCAACTCAGATTCTTGTGTGAAAATCTGCACGACGAAAGTATCGC
GACATTAGGTGACAGCAGTCGTGGCTGGGTGAATGATCCGACCTCTGCGGTCAACCTTCAACTAAACGAACTTATCGAGC
ATATTGCGGCATTTGTTGTTACCTACAAAATAAAATACCCGCATGAAGCGGCGTTATGTGAACGGGTTGAGAAATATCTG
GACGACACTTATATTCTTTTCAGCAATTACGGTATAAATGACTCCGAGTTGCAGAAATGGAAAAAATCCAAGTCACAATT
ATTCAGAATGTTCTCAGAAAAGAGCATCTGTACGGTAGTAAAAACTTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6