Detailed information of TA system TA224242

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3214094..3214878
• Replicon: chromosome
• Accession: NZ_CP086626
• Organism: Escherichia coli strain RIVM_C037134

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: LN362_RS15865
• Protein ID: WP_000613626.1
• Coordinates: 3214384..3214878 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: LN362_RS15860
• Protein ID: WP_001110447.1
• Coordinates: 3214094..3214387 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN362_RS15850 (3209244)	3209244..3210203	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
LN362_RS15855 (3210776)	3210776..3213961	+	3186	WP_001550951.1	ribonuclease E	-
LN362_RS15860 (3214094)	3214094..3214387	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
LN362_RS15865 (3214384)	3214384..3214878	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
LN362_RS15870 (3214973)	3214973..3215926	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
LN362_RS15875 (3215938)	3215938..3217581	-	1644	WP_000096478.1	flagellar hook-associated protein FlgK	-
LN362_RS15880 (3217647)	3217647..3218588	-	942	WP_001366226.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
LN362_RS15885 (3218588)	3218588..3219685	-	1098	WP_001441925.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T224242 WP_000613626.1 NZ_CP086626:3214384-3214878 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T224242 NZ_CP119773:c2603094-2602789 [Dickeya fangzhongdai]
ATGCAATTCATTGTTTATCAATACAAACGTGCCAGTCACTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGAGAC
ACCCAAGCGGCGCATGGTCATTCCGCTTATCGAATCACATCACCTGTCTGAGAAAGTGAATAAGACGTTGTTTCCTCTGC
TCCGCATCGACGGTGAAGATTATCGGCTGATGACCACTGAGTTGTCGAGCGTTCCAGTTGAGGTCATTGGTGAAGTGATT
GCGGATCTCAGCGACTGTGCGGATGAGATCAAGGATGCTATCAATCTTATGTTTTGGGGGATTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT224242 WP_001110447.1 NZ_CP086626:3214094-3214387 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT224242 NZ_CP119773:c2603315-2603097 [Dickeya fangzhongdai]
ATGAAACACCGCGTCAGCGTTACCGTGGACAAAGACAATTATCAGGTTCTGAGTGCTGCCGGAGTCAATATTTCCGGGCT
GGTGAATGATGCCATTGGCAAAGAAGCCCGCCGCATCAGGACTGAAGAGTGGAAGAAGGAAAACCGAGAAGGGATGGAAG
AGGTCGCCCGATTTATCGCGCAGCATGGCTCCTTTGCAGATGAAAACAGGAACTGGTGA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6