Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3902101..3902719 Replicon chromosome
Accession NZ_CP086618
Organism Escherichia coli strain RIVM_C039227

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LN355_RS19025 Protein ID WP_001291435.1
Coordinates 3902501..3902719 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LN355_RS19020 Protein ID WP_000344800.1
Coordinates 3902101..3902475 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN355_RS19010 (3897190) 3897190..3898383 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LN355_RS19015 (3898406) 3898406..3901555 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
LN355_RS19020 (3902101) 3902101..3902475 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LN355_RS19025 (3902501) 3902501..3902719 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LN355_RS19030 (3902891) 3902891..3903442 + 552 WP_000102564.1 maltose O-acetyltransferase -
LN355_RS19035 (3903558) 3903558..3904028 + 471 WP_000136192.1 YlaC family protein -
LN355_RS19040 (3904192) 3904192..3905742 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LN355_RS19045 (3905784) 3905784..3906137 - 354 WP_085334692.1 DUF1428 family protein -
LN355_RS19055 (3906516) 3906516..3906827 + 312 WP_000409911.1 MGMT family protein -
LN355_RS19060 (3906858) 3906858..3907430 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T224073 WP_001291435.1 NZ_CP086618:3902501-3902719 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T224073 NZ_CP119676:1098744-1098932 [Varunaivibrio sulfuroxidans]
ATGAACAGTAAAGAGATCATAAAGCGGCTCGAGTCCGATGGTTGGTTTGAAGTGCAACGGCGGGGATCGCATGTGCAGTT
TAAGCATGCCGCGAAAAAAGGACGGGTTACCGTCCCTCATCCCAAGCGTGACCTTCCGATCGGTACGCTTCGGAGCATCG
AGAAACAGGCCGGGGTCAAAATGAGGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT224073 WP_000344800.1 NZ_CP086618:3902101-3902475 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT224073 NZ_CP119676:1098938-1099342 [Varunaivibrio sulfuroxidans]
ATGGCACATTACATCGCGCTCTTGCGCAAAGAGGAAGAAAGCGACTTTGCCGTTGAATTTCCAGATTTTCCAGGGTGCGT
TACCGCCGGGACCACACTTGACGAGGCTAAGGATATGGCCGTCGAAGCGTTGGCTTTGCATATAGAGGGAATACGTGAAG
ATGGTGGAGGGCTTCCAACGCCTTCTCGTCTGGAAACCATCATGGCCGATCCTCACAATGTCGGGGCGGTTGCTTTCTTG
GTCGATGCGCCCGCAGTAAAGGAAAAAGCCGTGCGCGTGAACTTCACGATCCTCGAGAGCGTTCTACGCCGTGTTGACGC
CCATGCCAAGGCGCAGCATCTAACGCGGTCAGCGTTTTTGGCCGAGGCGGCAATCAAAGCGATAAGTGATAACGAGCACG
TCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References