Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 689665..690464 Replicon chromosome
Accession NZ_CP086616
Organism Escherichia coli strain RIVM_C039629

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag LN353_RS03375 Protein ID WP_000347270.1
Coordinates 689665..690129 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag LN353_RS03380 Protein ID WP_001551693.1
Coordinates 690129..690464 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN353_RS03345 (684666) 684666..685100 - 435 WP_000948837.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LN353_RS03350 (685118) 685118..685996 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LN353_RS03355 (685986) 685986..686765 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LN353_RS03360 (686776) 686776..687249 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LN353_RS03365 (687272) 687272..688552 - 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
LN353_RS03370 (688801) 688801..689610 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LN353_RS03375 (689665) 689665..690129 - 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LN353_RS03380 (690129) 690129..690464 - 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LN353_RS03385 (690613) 690613..692184 - 1572 WP_001273738.1 galactarate dehydratase -
LN353_RS03390 (692559) 692559..693893 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
LN353_RS03395 (693909) 693909..694679 + 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T224023 WP_000347270.1 NZ_CP086616:c690129-689665 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

>T224023 NZ_CP119571:c76131-75943 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT224023 WP_001551693.1 NZ_CP086616:c690464-690129 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT224023 NZ_CP119571:c76420-76160 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTATATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References