Detailed information of TA system TA223794

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 721937..722630
• Replicon: chromosome
• Accession: NZ_CP086556
• Organism: Escherichia coli strain ESY002

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: C5N07_RS03525
• Protein ID: WP_000415584.1
• Coordinates: 721937..722233 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: C5N07_RS03530
• Protein ID: WP_000650107.1
• Coordinates: 722235..722630 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5N07_RS03490 (717025)	717025..717339	-	315	WP_000958598.1	putative quinol monooxygenase	-
C5N07_RS03495 (717370)	717370..717951	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
C5N07_RS03500 (718270)	718270..718602	+	333	WP_000917685.1	DUF2645 family protein	-
C5N07_RS03505 (718648)	718648..719997	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
C5N07_RS03510 (719994)	719994..720653	-	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
C5N07_RS03515 (720805)	720805..721197	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
C5N07_RS03520 (721250)	721250..721732	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
C5N07_RS03525 (721937)	721937..722233	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
C5N07_RS03530 (722235)	722235..722630	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
C5N07_RS03535 (722763)	722763..724370	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
C5N07_RS03540 (724508)	724508..726766	+	2259	WP_032329482.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T223794 WP_000415584.1 NZ_CP086556:721937-722233 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T223794 NZ_CP119300:2357420-2358157 [Bacillus cereus]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACTGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGCTATTTTTGTTGATATTGCGTTC
ATTTTAACACCTAATATTTCTGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCCGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGATG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT223794 WP_000650107.1 NZ_CP086556:722235-722630 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT223794 NZ_CP119300:2358270-2358398 [Bacillus cereus]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65