Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3446406..3447024 Replicon chromosome
Accession NZ_CP086538
Organism Escherichia coli strain Ec-042-T5-MAC

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LNN52_RS16815 Protein ID WP_001291435.1
Coordinates 3446806..3447024 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LNN52_RS16810 Protein ID WP_000344800.1
Coordinates 3446406..3446780 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN52_RS16800 (3441495) 3441495..3442688 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LNN52_RS16805 (3442711) 3442711..3445860 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LNN52_RS16810 (3446406) 3446406..3446780 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LNN52_RS16815 (3446806) 3446806..3447024 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LNN52_RS16820 (3447196) 3447196..3447747 + 552 WP_000102568.1 maltose O-acetyltransferase -
LNN52_RS16825 (3447863) 3447863..3448333 + 471 WP_000136192.1 YlaC family protein -
LNN52_RS16830 (3448497) 3448497..3450047 + 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LNN52_RS16835 (3450089) 3450089..3450442 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LNN52_RS16845 (3450821) 3450821..3451132 + 312 WP_000409911.1 MGMT family protein -
LNN52_RS16850 (3451163) 3451163..3451735 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T223757 WP_001291435.1 NZ_CP086538:3446806-3447024 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T223757 NZ_CP119176:4695-4961 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT223757 WP_000344800.1 NZ_CP086538:3446406-3446780 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT223757 NZ_CP119176:4487-4711 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References