Detailed information of TA system TA022357

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/Txe-RelB
• Location: 2060728..2061257
• Replicon: chromosome
• Accession: NC_009727
• Organism: Coxiella burnetii Dugway 5J108-111

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q83AB3
• Locus tag: CBUD_RS10410
• Protein ID: WP_005769718.1
• Coordinates: 2060982..2061257 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: Q83AB4
• Locus tag: CBUD_RS10405
• Protein ID: WP_005769716.1
• Coordinates: 2060728..2060982 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CBUD_RS10370	2055791..2056165	+	375	WP_011997407.1	hypothetical protein	-
CBUD_RS10375	2056210..2056908	+	699	WP_011997408.1	outer membrane beta-barrel protein	-
CBUD_RS10380	2056972..2057913	+	942	WP_005769709.1	S49 family peptidase	-
CBUD_RS10385	2057926..2058801	+	876	WP_011997409.1	symmetrical bis(5'-nucleosyl)-tetraphosphatase	-
CBUD_RS12370	2058777..2058890	+	114	Protein_2032	type II toxin-antitoxin system YoeB family toxin	-
CBUD_RS10390	2058950..2059084	+	135	WP_010958583.1	hypothetical protein	-
CBUD_RS10395	2059250..2060267	+	1018	Protein_2034	IS110-like element IS1111A family transposase	-
CBUD_RS10400	2060264..2060536	-	273	WP_010957319.1	hypothetical protein	-
CBUD_RS10405	2060728..2060982	+	255	WP_005769716.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
CBUD_RS10410	2060982..2061257	+	276	WP_005769718.1	Txe/YoeB family addiction module toxin	Toxin
CBUD_RS10415	2061230..2061715	-	486	WP_005769725.1	dihydrofolate reductase	-
CBUD_RS10420	2061712..2062515	-	804	WP_011997410.1	epoxyqueuosine reductase QueH	-
CBUD_RS10425	2062711..2062998	+	288	WP_005769730.1	acylphosphatase	-
CBUD_RS10430	2062974..2063528	-	555	WP_005769732.1	D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase	-
CBUD_RS10435	2063541..2064485	-	945	WP_010958586.1	methionyl-tRNA formyltransferase	-
CBUD_RS10440	2064673..2065599	+	927	WP_011997412.1	DNA-processing protein DprA	-
CBUD_RS10445	2065596..2066075	+	480	WP_011997413.1	DUF494 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 11150.86 Da
• Isoelectric Point: 9.6762

>T22357 WP_005769718.1 NC_009727:2060982-2061257 [Coxiella burnetii Dugway 5J108-111]
MQISFTPEAWEDYLYWQKFDKKMLRRINELIKDAMHEPFSGKGKPEPLKFELQGYWSRRLDQEHRLVYKVLDDSLMIIAA
RFHYNRLNSKN

Nucleotide

• Length: 276 bp

>T22357 NC_009727:2060982-2061257 [Coxiella burnetii Dugway 5J108-111]
ATGCAAATTTCCTTCACGCCCGAAGCCTGGGAAGATTATTTATATTGGCAAAAATTCGACAAAAAAATGCTTCGACGCAT
TAATGAACTCATTAAGGATGCTATGCACGAGCCTTTTTCCGGAAAGGGAAAGCCAGAACCTTTAAAATTTGAATTACAAG
GATATTGGTCAAGACGATTAGATCAAGAACATCGATTGGTCTACAAAGTTTTAGACGATTCGTTAATGATTATCGCCGCA
AGATTTCACTATAATCGCCTTAATTCTAAAAACTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9624.25 Da
• Isoelectric Point: 10.4802

>AT22357 WP_005769716.1 NC_009727:2060728-2060982 [Coxiella burnetii Dugway 5J108-111]
MNVVTFSELRAQLKKILDLSADQHEPVVVKRPNKETMVILSLRDFEALKETAYLLSNEANAARLRQSIRSLKQGKAQKKK
LMED

Nucleotide

• Length: 255 bp

>AT22357 NC_009727:2060728-2060982 [Coxiella burnetii Dugway 5J108-111]
ATGAACGTAGTTACTTTTAGCGAACTCAGGGCGCAACTTAAAAAAATCTTGGATCTTTCCGCCGATCAACACGAACCTGT
CGTCGTTAAACGGCCAAATAAAGAAACCATGGTCATTTTATCTTTACGCGACTTTGAGGCTCTAAAAGAAACAGCTTATC
TCTTAAGTAACGAAGCTAATGCGGCCCGTCTTCGTCAGTCTATCCGCAGCTTAAAACAAGGCAAGGCACAAAAAAAGAAA
TTAATGGAAGATTAA

Structures

Toxin

• AlphaFold DB: Q83AB3

Antitoxin

• AlphaFold DB: Q83AB4