Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4243793..4244388 Replicon chromosome
Accession NZ_CP086470
Organism Escherichia coli strain Ec-050-T20-ESBL

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag LNN47_RS20410 Protein ID WP_000239579.1
Coordinates 4243793..4244143 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag LNN47_RS20415 Protein ID WP_001223208.1
Coordinates 4244137..4244388 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN47_RS20390 (4239239) 4239239..4240261 - 1023 WP_001295197.1 ABC transporter permease -
LNN47_RS20395 (4240275) 4240275..4241777 - 1503 WP_000205784.1 sugar ABC transporter ATP-binding protein -
LNN47_RS20400 (4241917) 4241917..4242873 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
LNN47_RS20405 (4243183) 4243183..4243713 + 531 WP_000055072.1 inorganic diphosphatase -
LNN47_RS20410 (4243793) 4243793..4244143 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
LNN47_RS20415 (4244137) 4244137..4244388 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
LNN47_RS20420 (4244600) 4244600..4244941 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
LNN47_RS20425 (4244944) 4244944..4248723 - 3780 WP_000060978.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T223475 WP_000239579.1 NZ_CP086470:c4244143-4243793 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T223475 NZ_CP118902:c78715-78245 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACAGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT223475 WP_001223208.1 NZ_CP086470:c4244388-4244137 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT223475 NZ_CP118902:c79155-78712 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAAGTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACTGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References