Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4012317..4012914 Replicon chromosome
Accession NZ_CP086447
Organism Klebsiella pneumoniae strain AE-2247421-T0

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag LNN40_RS19705 Protein ID WP_004142563.1
Coordinates 4012597..4012914 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag LNN40_RS19700 Protein ID WP_004142561.1
Coordinates 4012317..4012604 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN40_RS19670 (4008398) 4008398..4008646 + 249 WP_002893037.1 DUF1158 domain-containing protein -
LNN40_RS19675 (4008664) 4008664..4009005 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
LNN40_RS19680 (4009036) 4009036..4010151 - 1116 Protein_3874 MBL fold metallo-hydrolase -
LNN40_RS19685 (4010330) 4010330..4010911 + 582 WP_032419262.1 TetR/AcrR family transcriptional regulator -
LNN40_RS19690 (4010911) 4010911..4011279 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
LNN40_RS19695 (4011399) 4011399..4012052 + 654 WP_032419263.1 oxygen-insensitive NAD(P)H nitroreductase -
LNN40_RS19700 (4012317) 4012317..4012604 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
LNN40_RS19705 (4012597) 4012597..4012914 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LNN40_RS19710 (4013099) 4013099..4014142 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
LNN40_RS19715 (4014809) 4014809..4015675 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
LNN40_RS19720 (4015784) 4015784..4017211 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T223345 WP_004142563.1 NZ_CP086447:c4012914-4012597 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T223345 NZ_CP118723:4326286-4326576 [Serratia marcescens]
ATGATTAAATCTTTCCGCGATCGCTATCTGGAGCAGTTCTATCTGGAAGGCAGGCGCAGCCGTCTGATCCCCGGCGCGCT
TGAGCGACAGTTGGCCAGAAAACTCGATATGTTGGCCGCCGCGCAGCAAGAGCGTGATTTGCATCACCCCACGGGTAACT
ATTACAAGCGGCTATCCGGACCATTTCAGGGATGGTCAAGTCTGCGCGTCAATATGCACTGGCGGCTGATGTTCCAGTGG
CGGCATGATGCCGCTGAAAAAATTTACCTCGATCCTCATCAAGATACCTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT223345 WP_004142561.1 NZ_CP086447:c4012604-4012317 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT223345 NZ_CP118723:4326591-4326914 [Serratia marcescens]
ATGTTGGAAACCACGACTCGCGAACCCACCACGGTGGGAGAAGTGCTGCAGGAAGAATATTTAACGCCGCTAGGCATCGG
CCAGGAGGAACTGGCAAAGGTATTGGGCGTCACCCGCGCCACCGTCAATCGCCTGTGCAACGGGCATCGACGCATGAGCG
TTGCCGAGGCCAATCTGTTTGCCGATCTGTTCGAAACGACCGCTGACTTTTGGCTAAACCTGCAGGCGGCGCACGATCGC
TGGGAAGCCAGACAGGCCGACGCGCAGCGTGTGCGGCTGCGGCCGATCAAAGAAATACTGAGGCGCAATATCACTCATGC
ATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References