Detailed information of TA system TA022316

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ygfYX/Cpta(toxin)
• Location: 1477890..1478559
• Replicon: chromosome
• Accession: NC_009665
• Organism: Shewanella baltica OS185

Toxin (Protein)

• Gene name: ygfX
• Uniprot ID: A9L5M8
• Locus tag: SHEW185_RS06375
• Protein ID: WP_012088602.1
• Coordinates: 1478119..1478559 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: ygfY
• Uniprot ID: -
• Locus tag: SHEW185_RS06370
• Protein ID: WP_006080770.1
• Coordinates: 1477890..1478138 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SHEW185_RS06345	1473546..1474355	+	810	WP_012088600.1	prolipoprotein diacylglyceryl transferase	-
SHEW185_RS06350	1474352..1475146	+	795	WP_006085062.1	thymidylate synthase	-
SHEW185_RS06355	1475264..1476433	+	1170	WP_006080767.1	Na+/H+ antiporter NhaA	-
SHEW185_RS06360	1476436..1476822	+	387	WP_006080768.1	hypothetical protein	-
SHEW185_RS06365	1476896..1477804	+	909	WP_006085064.1	transcriptional activator NhaR	-
SHEW185_RS06370	1477890..1478138	+	249	WP_006080770.1	succinate dehydrogenase assembly factor 2	Antitoxin
SHEW185_RS06375	1478119..1478559	+	441	WP_012088602.1	hypothetical protein	Toxin
SHEW185_RS06380	1478619..1480232	-	1614	WP_006080772.1	L-aspartate oxidase	-
SHEW185_RS06385	1480422..1481000	+	579	WP_006080773.1	RNA polymerase sigma factor RpoE	-
SHEW185_RS06390	1481042..1481668	+	627	WP_006080774.1	anti-sigma factor	-
SHEW185_RS06395	1481681..1482613	+	933	WP_006080775.1	MucB/RseB C-terminal domain-containing protein	-
SHEW185_RS06400	1482624..1483097	+	474	WP_006080776.1	SoxR reducing system RseC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (6-135)

Antitoxin

• Flavinator of succinate dehydrogenase (7-77)

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 17252.42 Da
• Isoelectric Point: 8.3668

>T22316 WP_012088602.1 NC_009665:1478119-1478559 [Shewanella baltica OS185]
VEDRHHSFSVKASFDQRLSLVVFICVCASSFLLWPQSDNLALSLLKYLFILLVCIFLLSQLWRLQHWRLDFVLSDKGVGR
LSTGEHFQVLKRTWVTPFVCLMYIEVDTQLRLLMVWADMLDDTDYRHLCRLLLRAKIKQTKPHTEI

Nucleotide

• Length: 441 bp

>T22316 NC_009665:1478119-1478559 [Shewanella baltica OS185]
GTGGAAGACCGGCACCATAGTTTCAGCGTAAAAGCCTCCTTCGACCAACGACTCTCGTTGGTCGTTTTTATTTGTGTCTG
TGCTAGCTCATTTCTGCTCTGGCCTCAAAGCGACAATCTCGCCTTATCCCTGCTTAAATATCTGTTTATCCTGCTAGTTT
GTATTTTCTTGCTATCACAGTTGTGGCGACTGCAGCATTGGCGTTTAGATTTTGTGCTTAGTGATAAAGGCGTGGGACGT
TTGTCAACGGGTGAGCACTTTCAGGTATTAAAGCGAACTTGGGTGACGCCCTTTGTTTGCTTGATGTACATCGAAGTGGA
TACTCAGTTACGGCTCTTGATGGTTTGGGCGGATATGTTAGACGATACTGACTATCGGCATTTATGTCGGCTGTTGCTGC
GGGCTAAAATAAAACAGACTAAACCTCATACCGAGATTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9723.36 Da
• Isoelectric Point: 4.4929

>AT22316 WP_006080770.1 NC_009665:1477890-1478138 [Shewanella baltica OS185]
MELMNIARVRWACRRGMLELDVLFQPFVENFYQEMSDEDKTVFIRLLECEDPELFAWFMGHEVCPDAELARMVVKVRGRP
AP

Nucleotide

• Length: 249 bp

>AT22316 NC_009665:1477890-1478138 [Shewanella baltica OS185]
TTGGAATTAATGAACATCGCAAGGGTTCGCTGGGCATGTCGCCGCGGGATGCTAGAATTAGACGTTTTGTTTCAACCTTT
TGTTGAAAATTTCTATCAAGAAATGTCCGATGAAGATAAAACTGTGTTTATCCGTTTGTTGGAATGTGAAGATCCCGAAT
TGTTTGCATGGTTTATGGGCCATGAAGTGTGCCCTGATGCTGAGCTCGCGCGTATGGTAGTGAAAGTCCGTGGAAGACCG
GCACCATAG

Structures

Toxin

• AlphaFold DB: A9L5M8