Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 75929..76530 Replicon plasmid p1585m1_C
Accession NZ_CP086387
Organism Escherichia coli strain 1585m1

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag LM401_RS00415 Protein ID WP_001216034.1
Coordinates 75929..76309 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag LM401_RS00420 Protein ID WP_001190712.1
Coordinates 76309..76530 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LM401_RS00375 (LM401_00375) 70996..71130 - 135 WP_215243272.1 phage antirepressor KilAC domain-containing protein -
LM401_RS00380 (LM401_00380) 71448..71798 - 351 Protein_75 ORF6N domain-containing protein -
LM401_RS00385 (LM401_00385) 72107..72328 - 222 WP_000246821.1 host cell division inhibitor Icd-like protein -
LM401_RS00390 (LM401_00390) 72347..72466 - 120 WP_001376634.1 ash family protein -
LM401_RS00395 (LM401_00395) 72688..73815 - 1128 WP_001446093.1 hypothetical protein -
LM401_RS00400 (LM401_00400) 73879..74414 + 536 Protein_79 IS3-like element IS2 family transposase -
LM401_RS00405 (LM401_00405) 74674..75717 - 1044 WP_000648817.1 DUF968 domain-containing protein -
LM401_RS00410 (LM401_00410) 75745..75924 - 180 WP_001446091.1 hypothetical protein -
LM401_RS00415 (LM401_00415) 75929..76309 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LM401_RS00420 (LM401_00420) 76309..76530 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LM401_RS00425 (LM401_00425) 76713..78269 + 1557 WP_001568002.1 type I restriction-modification system subunit M -
LM401_RS00430 (LM401_00430) 78266..79501 + 1236 WP_032175094.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..107575 107575


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T223148 WP_001216034.1 NZ_CP086387:c76309-75929 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T223148 NZ_CP118539:1200642-1200947 [Xylella fastidiosa]
ATGAATAATGAGACTTTTAGTCGATACGACACCGCTGACTATCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCTGTCGCTCGTGCTCGTAATTTAA
GTCAGCTTGCTCGTGACGTTGGCATGTCCAGGCAAGGACTAGACAAGGCACTCTCTAACGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTGGCTAAAGCGCTTGGTTTACGGATGTCATTCACACCTTCCTCTATGAGTTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT223148 WP_001190712.1 NZ_CP086387:c76530-76309 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT223148 NZ_CP118539:1200342-1200638 [Xylella fastidiosa]
ATGGTCGAACTCATCAAAACCAGCACTTTTGATGCTTGGATAAACAGCTTACGTGACCGCAAAGCAGCCGCCAGAATTCA
AGCGCGTCTTGATCGGCTCGCACTTGGCAATCCCGGCGACGTGAAGCCTGTGGGTGCTGGCATCTCAGAAATGAGGATCG
ACCATGGGCCCGGATATCGGATCTATTTTATGAAGCATGGTGCAGTACTGATTCTCTTACTTTGCGGTGGCGATAAATCG
TCACAAGTACGAGATATAGAGCAGGCTAAGGCACTGGCTGCACTGTGGAAGGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References