Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/ParE-RHH
Location 2776837..2777357 Replicon chromosome
Accession NZ_CP086348
Organism Clavibacter michiganensis subsp. nebraskensis strain SL1

Toxin (Protein)


Gene name parE Uniprot ID M5BDX6
Locus tag LIX21_RS13115 Protein ID WP_015491236.1
Coordinates 2776837..2777127 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A399PXI2
Locus tag LIX21_RS13120 Protein ID WP_041465310.1
Coordinates 2777124..2777357 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIX21_RS13105 (LIX21_002620) 2774786..2776384 + 1599 WP_015491235.1 class I SAM-dependent methyltransferase -
LIX21_RS13110 (LIX21_002622) 2776543..2776797 + 255 Protein_2577 helix-turn-helix transcriptional regulator -
LIX21_RS13115 (LIX21_002623) 2776837..2777127 - 291 WP_015491236.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LIX21_RS13120 (LIX21_002624) 2777124..2777357 - 234 WP_041465310.1 type II toxin-antitoxin system ParD family antitoxin Antitoxin
LIX21_RS13125 (LIX21_002625) 2777469..2777669 - 201 WP_015491238.1 hypothetical protein -
LIX21_RS13130 (LIX21_002626) 2777685..2777996 - 312 WP_236685126.1 antitoxin VbhA family protein -
LIX21_RS13135 (LIX21_002627) 2777995..2779068 + 1074 WP_155119035.1 LD-carboxypeptidase -
LIX21_RS13140 (LIX21_002628) 2779245..2779604 + 360 WP_015491241.1 hypothetical protein -
LIX21_RS13145 (LIX21_002629) 2779765..2780280 + 516 WP_041465311.1 hypothetical protein -
LIX21_RS13150 (LIX21_002630) 2780427..2781464 + 1038 WP_015491244.1 DUF1266 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-92)

Antitoxin

Antitoxin ParD

(4-72)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11338.63 Da        Isoelectric Point: 6.4832

>T223103 WP_015491236.1 NZ_CP086348:c2777127-2776837 [Clavibacter michiganensis subsp. nebraskensis]
VKPHRLTPAATKDLSEIWDYTEERWDADQAETYVREIGAAIRRVAEDPTRGRDCGDIRPGYRRYSIESHTVYYVDRPETV
DVIRILHQRMDPSRHL

Download         Length: 291 bp

>T223103 NZ_CP118495:472200-472550 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8268.18 Da        Isoelectric Point: 4.4000

>AT223103 WP_041465310.1 NZ_CP086348:c2777357-2777124 [Clavibacter michiganensis subsp. nebraskensis]
MAQNTSISLDGHFTGFLAREVESGRYRSASEVVRAGLRLLEDQETQLEALRAALVAGEASGEAAPFDLDAFIADKRA

Download         Length: 234 bp

>AT223103 NZ_CP118495:471913-472194 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M5BDX6

Antitoxin

Source ID Structure
AlphaFold DB A0A399PXI2

References