Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1302633..1303251 Replicon chromosome
Accession NZ_CP086342
Organism Escherichia coli strain ESY001

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C5N06_RS06285 Protein ID WP_001291435.1
Coordinates 1302633..1302851 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C5N06_RS06290 Protein ID WP_000344800.1
Coordinates 1302877..1303251 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N06_RS06250 (1297922) 1297922..1298494 + 573 WP_000779831.1 YbaY family lipoprotein -
C5N06_RS06255 (1298525) 1298525..1298836 - 312 WP_000409911.1 MGMT family protein -
C5N06_RS06265 (1299215) 1299215..1299568 + 354 WP_000878140.1 DUF1428 domain-containing protein -
C5N06_RS06270 (1299610) 1299610..1301160 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C5N06_RS06275 (1301324) 1301324..1301794 - 471 WP_000136192.1 YlaC family protein -
C5N06_RS06280 (1301910) 1301910..1302461 - 552 WP_000102564.1 maltose O-acetyltransferase -
C5N06_RS06285 (1302633) 1302633..1302851 - 219 WP_001291435.1 HHA domain-containing protein Toxin
C5N06_RS06290 (1302877) 1302877..1303251 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C5N06_RS06295 (1303797) 1303797..1306946 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
C5N06_RS06300 (1306969) 1306969..1308162 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T223082 WP_001291435.1 NZ_CP086342:c1302851-1302633 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T223082 NZ_CP118399:50668-51150 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT223082 WP_000344800.1 NZ_CP086342:c1303251-1302877 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT223082 NZ_CP118399:50414-50680 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References