Detailed information of TA system TA223070

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2433230..2433868
• Replicon: chromosome
• Accession: NZ_CP086340
• Organism: Escherichia coli strain ESY003

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: C5N08_RS11655
• Protein ID: WP_001447010.1
• Coordinates: 2433692..2433868 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C5N08_RS11650
• Protein ID: WP_001270286.1
• Coordinates: 2433230..2433646 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5N08_RS11630 (2428382)	2428382..2429323	-	942	WP_001251304.1	ABC transporter permease	-
C5N08_RS11635 (2429324)	2429324..2430337	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
C5N08_RS11640 (2430355)	2430355..2431500	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
C5N08_RS11645 (2431745)	2431745..2433151	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
C5N08_RS11650 (2433230)	2433230..2433646	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
C5N08_RS11655 (2433692)	2433692..2433868	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
C5N08_RS11660 (2434090)	2434090..2434320	+	231	WP_000494244.1	YncJ family protein	-
C5N08_RS11665 (2434412)	2434412..2436373	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
C5N08_RS11670 (2436446)	2436446..2436982	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
C5N08_RS11675 (2437074)	2437074..2438249	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T223070 WP_001447010.1 NZ_CP086340:c2433868-2433692 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T223070 NZ_CP118398:835232-835642 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT223070 WP_001270286.1 NZ_CP086340:c2433646-2433230 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT223070 NZ_CP118398:834985-835251 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8