Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3940445..3941172 Replicon chromosome
Accession NZ_CP086336
Organism Escherichia coli strain EBJ001

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag C5N11_RS19040 Protein ID WP_000550189.1
Coordinates 3940858..3941172 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag C5N11_RS19035 Protein ID WP_000560266.1
Coordinates 3940445..3940861 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N11_RS19025 (3935605) 3935605..3937956 + 2352 WP_000695487.1 alpha-glucosidase -
C5N11_RS19030 (3938382) 3938382..3940400 + 2019 WP_000121435.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
C5N11_RS19035 (3940445) 3940445..3940861 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
C5N11_RS19040 (3940858) 3940858..3941172 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
C5N11_RS19045 (3941456) 3941456..3942592 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
C5N11_RS19050 (3942677) 3942677..3943180 + 504 WP_001333820.1 M48 family metallopeptidase -
C5N11_RS19055 (3943257) 3943257..3943949 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
C5N11_RS19060 (3944028) 3944028..3945014 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T223028 WP_000550189.1 NZ_CP086336:c3941172-3940858 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T223028 NZ_CP118277:c4618045-4617671 [Klebsiella quasipneumoniae]
ATGCAAAAACAATCCCTGTCCTCTCATAGGGCGGCCTCGTCACGTCTGTCATCAATCGAAATCTGGCGAAAATTACTGAA
GTATCTGCTGGAGCAACACTACGGCCTTACCCTCAACGACACACCGTTTGGCAACGACAGTGTGATCCAGAAGCACATTG
ATGCGGGAATCTCACTGTGTGATGCGGTGAATTTTATCGTGGAAAAGTACGACCTGGTGCGTACTGACCGTCACGGCTTC
AGCGTGAAAGAGCAGTCTCCGTTTATCGGCAGCATTGATATGCTCCGCGCCCGGAAGGCTACCGGGCTGATGACCCGCAA
GGGATATAAAACCGTTACTGACATCACAGGTGACAGATTCAGCGGAGGGAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT223028 WP_000560266.1 NZ_CP086336:c3940861-3940445 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT223028 NZ_CP118277:c4618454-4618095 [Klebsiella quasipneumoniae]
ATGAAGAAAGCAACCCGTGTAATCAACCATAACATCGCGGAACCCTGGTGGGGCCTCAGACGCAACATCACGCCGTGTTT
GGGTGCCCGGCTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCTGACCGCGCCAGCATTGCCGGGACATTTAATGACG
CAGATTTACGCCATCTGGACCAGGCATTTCCGGTACTGATGAAACAGATGGAGCTTATGCTGGCCAGCAGAGAACTCACG
CCCCACATTCAGCGCTGCATTACGCTCCACGCAAAAGGGCTGATATGTGAGGCCGATACGCTCGGCTCCTGTGGCTATCT
GTACATCGTAATTTATCCGACATCTGCAACAACCGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References