Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4244377..4245211 Replicon chromosome
Accession NZ_CP086334
Organism Escherichia coli strain EBJ003

Toxin (Protein)


Gene name yeeV Uniprot ID A0A2T3TJB4
Locus tag C5N12_RS20575 Protein ID WP_000854811.1
Coordinates 4244377..4244754 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2T3TJJ3
Locus tag C5N12_RS20580 Protein ID WP_001285590.1
Coordinates 4244843..4245211 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N12_RS20555 (4242175) 4242175..4243395 + 1221 WP_000794251.1 capsular biosynthesis protein -
C5N12_RS20560 (4243421) 4243421..4243651 - 231 Protein_4013 hypothetical protein -
C5N12_RS20565 (4243757) 4243757..4243933 - 177 WP_000839288.1 DUF957 domain-containing protein -
C5N12_RS20570 (4243950) 4243950..4244380 - 431 Protein_4015 DUF5983 family protein -
C5N12_RS20575 (4244377) 4244377..4244754 - 378 WP_000854811.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
C5N12_RS20580 (4244843) 4244843..4245211 - 369 WP_001285590.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
C5N12_RS20585 (4245285) 4245285..4245506 - 222 WP_000692300.1 DUF987 domain-containing protein -
C5N12_RS20590 (4245575) 4245575..4246051 - 477 WP_001186725.1 RadC family protein -
C5N12_RS20595 (4246067) 4246067..4246552 - 486 WP_064770836.1 antirestriction protein -
C5N12_RS20600 (4246644) 4246644..4247462 - 819 WP_229019971.1 DUF932 domain-containing protein -
C5N12_RS20605 (4247580) 4247580..4247751 - 172 Protein_4022 DUF905 family protein -
C5N12_RS25805 (4247706) 4247706..4247834 - 129 WP_001305405.1 hypothetical protein -
C5N12_RS20610 (4247852) 4247852..4248307 - 456 WP_000581502.1 IrmA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14041.14 Da        Isoelectric Point: 7.8839

>T223003 WP_000854811.1 NZ_CP086334:c4244754-4244377 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Download         Length: 378 bp

>T223003 NZ_CP118273:3965222-3965440 [Klebsiella quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13608.50 Da        Isoelectric Point: 6.6242

>AT223003 WP_001285590.1 NZ_CP086334:c4245211-4244843 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLYSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT223003 NZ_CP118273:3964821-3965195 [Klebsiella quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2T3TJB4

Antitoxin

Source ID Structure
AlphaFold DB A0A2T3TJJ3

References