Detailed information of TA system TA222988

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 822382..823020
• Replicon: chromosome
• Accession: NZ_CP086334
• Organism: Escherichia coli strain EBJ003

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: C5N12_RS04205
• Protein ID: WP_001306887.1
• Coordinates: 822844..823020 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C5N12_RS04200
• Protein ID: WP_001270286.1
• Coordinates: 822382..822798 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5N12_RS04180 (817534)	817534..818475	-	942	WP_001251315.1	ABC transporter permease	-
C5N12_RS04185 (818476)	818476..819489	-	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
C5N12_RS04190 (819507)	819507..820652	-	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
C5N12_RS04195 (820897)	820897..822303	-	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
C5N12_RS04200 (822382)	822382..822798	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
C5N12_RS04205 (822844)	822844..823020	-	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
C5N12_RS04210 (823242)	823242..823472	+	231	WP_000494241.1	YncJ family protein	-
C5N12_RS04215 (823564)	823564..825525	-	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
C5N12_RS04220 (825598)	825598..826134	-	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
C5N12_RS04225 (826226)	826226..827398	+	1173	WP_001523225.1	BenE family transporter YdcO	-
C5N12_RS04230 (827506)	827506..827964	-	459	WP_000526135.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	827506..827964	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T222988 WP_001306887.1 NZ_CP086334:c823020-822844 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T222988 NZ_CP118271:802360-802770 [Klebsiella quasipneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCTCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGCTGGTTCCCTGGCCGTTAAGCTACACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTTGACTGTG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGGCTGCGCTGGCAAAACGCC
GACTGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT222988 WP_001270286.1 NZ_CP086334:c822798-822382 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT222988 NZ_CP118271:802113-802379 [Klebsiella quasipneumoniae]
ATGGACATTAACAATAAAGCTCGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TATTCAACTGGCTGATGAACCATGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76