Detailed information of TA system TA222756

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 5032177..5033435
• Replicon: chromosome
• Accession: NZ_CP086213
• Organism: Pseudomonas aeruginosa strain Pa3

Toxin (Protein)

• Gene name: -
• Uniprot ID: V6AJ34
• Locus tag: LLJ53_RS23720
• Protein ID: WP_003086331.1
• Coordinates: 5032177..5032932 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: LLJ53_RS23725
• Protein ID: WP_003086329.1
• Coordinates: 5033067..5033435 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLJ53_RS23710 (LLJ53_23705)	5027348..5029891	+	2544	WP_033955245.1	acyl-homoserine-lactone acylase QuiP	-
LLJ53_RS23715 (LLJ53_23710)	5030047..5031408	-	1362	WP_003120632.1	DNA recombination protein RmuC	-
LLJ53_RS23720 (LLJ53_23715)	5032177..5032932	-	756	WP_003086331.1	RES family NAD+ phosphorylase	Toxin
LLJ53_RS23725 (LLJ53_23720)	5033067..5033435	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
LLJ53_RS23730 (LLJ53_23725)	5033508..5034794	-	1287	WP_003086327.1	FAD-binding oxidoreductase	-
LLJ53_RS23735 (LLJ53_23730)	5034850..5036343	-	1494	WP_015648322.1	aldehyde dehydrogenase family protein	-
LLJ53_RS23740 (LLJ53_23735)	5036518..5036994	+	477	WP_004353334.1	elongation factor GreAB	-
LLJ53_RS23745 (LLJ53_23740)	5036997..5037272	-	276	WP_023088486.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28236.34 Da
• Isoelectric Point: 7.7294

>T222756 WP_003086331.1 NZ_CP086213:c5032932-5032177 [Pseudomonas aeruginosa]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T222756 NZ_CP118135:1974296-1974724 [Pseudomonas chlororaphis]
ATGATCGTGCTCGACACCAATGTGCTGTCGGAACTGATGCGTCCCCAACCGGACGCATCGGTATTGCTGTGGATCGAAGC
GCAACCTGTCGAAGACCTCTACATCAGCGCCATGACCATGGCCGAGATTCTTCACGGCATCGCGCGCCTGCCCCACGGCA
GGCGCAAACAGATGCTCCATGCGTCGGCCCTGGCGATGTTCGAGGAAGATTTCATCGAACGCATCGTACCCTTCGGGACC
CAGGCGGCTCTTTACTACGCCCATCTGATTTCGCACCGGGAAAGCCTGGGGCGTCCGATCAGCCTGGTGGATGCGCAAAT
TGCGGCCATATGCCGCGTCCATGACGCGGCGATCGCCACCCGTAACGCCAAGGACTTCAGCGACACCGGGTTGATCGTTA
TCAACCCCTGGCTGCCACTCGAGGTTTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT222756 WP_003086329.1 NZ_CP086213:c5033435-5033067 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT222756 NZ_CP118135:1974054-1974299 [Pseudomonas chlororaphis]
ATGGCCAGCCTGACAATCCGAAACCTGGACGACCACATCAAAGAGCGCCTGCGCATCGAGGCCGCCCACAACGGTCACTC
CATGGAAGAAGAAGCCCGCTTGATTTTGCGCCGCGCCCTGAACAGAACAACCGACAGCCAAGGCCTGGCCAGTCGTATCC
GGGCACGCTTTGCCGAGGCCGGCGGAGTGAAGCTTGAGCTGCCCGAACGTACCGAGCCGGCACGCGTGGTGGATCTGGGC
GAATGA

Structures

Toxin

• AlphaFold DB: V6AJ34

Antitoxin

• AlphaFold DB: V6AJU7