Detailed information of TA system TA222530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 5719..6740
• Replicon: plasmid pSM-2
• Accession: NZ_CP086108
• Organism: Skermanella mucosa strain KEMB 2255-438

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: JL100_RS33030
• Protein ID: WP_228421747.1
• Coordinates: 5719..6291 (-)
• Length: 191 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: JL100_RS33035
• Protein ID: WP_202683336.1
• Coordinates: 6291..6740 (-)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL100_RS33020 (JL100_033110)	1766..4540	+	2775	WP_202683334.1	formate dehydrogenase subunit alpha	-
JL100_RS33025 (JL100_033115)	4884..5711	+	828	WP_228421718.1	IclR family transcriptional regulator C-terminal domain-containing protein	-
JL100_RS33030 (JL100_033120)	5719..6291	-	573	WP_228421747.1	PIN domain-containing protein	Toxin
JL100_RS33035 (JL100_033125)	6291..6740	-	450	WP_202683336.1	helix-turn-helix domain-containing protein	Antitoxin
JL100_RS33040 (JL100_033130)	7044..7346	-	303	WP_202683337.1	helix-turn-helix transcriptional regulator	-
JL100_RS33045 (JL100_033135)	7385..7843	-	459	WP_202683338.1	type II toxin-antitoxin system VapC family toxin	-
JL100_RS33050 (JL100_033140)	7891..8133	-	243	WP_202683339.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
JL100_RS33055 (JL100_033145)	8725..10074	+	1350	WP_228421149.1	IS1380 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..434629	434629
	-	flank	IS/Tn	-	-	8725..10074	1349

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-114)

Antitoxin

• SinI-like, DNA-binding domain (79-126)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 21351.54 Da
• Isoelectric Point: 6.2923

>T222530 WP_228421747.1 NZ_CP086108:c6291-5719 [Skermanella mucosa]
ILAGFTVVYDACVLYPAPLRDLLIELAAANLFRAKWTDAIHDEWIRNLLSNRPDLGIERLRRTRELMNTAVLDCLVKGYE
HLTPAVSLPDEDDRHVVAAAIHSRADAIVTFNLKDFPASELSRHDLEAVHPDEFIQHLFELDGAAVVIAAQRCHRKLKNP
PKSAGEYLDTLLRQSLPKTVASLRPFSEVI

Nucleotide

• Length: 573 bp

>T222530 NZ_CP117959:4689258-4689545 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 16689.03 Da
• Isoelectric Point: 5.6560

>AT222530 WP_202683336.1 NZ_CP086108:c6740-6291 [Skermanella mucosa]
MNALIRRPESVVPTDEDTRLAAELSRVLAANGENAALRVQLDDGRALTLPMAATRLLEQVLTEMAHGNAVTLIPVHAELT
TQEAADYLNVSRPYLVRLLEENFIPFHKVGTHRRVRFQDLKTFKEQADAKRRDAMGELVAQAQELDLGY

Nucleotide

• Length: 450 bp

>AT222530 NZ_CP117959:4689532-4689834 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA