Detailed information of TA system TA222480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 4915904..4916403
• Replicon: chromosome
• Accession: NZ_CP086069
• Organism: Rhodococcus pyridinivorans strain 4-4

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LLA01_RS22880
• Protein ID: WP_252173440.1
• Coordinates: 4915904..4916158 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: H0JMW3
• Locus tag: LLA01_RS22885
• Protein ID: WP_006550855.1
• Coordinates: 4916155..4916403 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLA01_RS22850 (LLA01_22885)	4911337..4912506	-	1170	WP_019290521.1	acyl-CoA dehydrogenase family protein	-
LLA01_RS22855 (LLA01_22890)	4912609..4913235	+	627	WP_006550849.1	TetR family transcriptional regulator	-
LLA01_RS22860 (LLA01_22895)	4913393..4913605	-	213	WP_060652491.1	hypothetical protein	-
LLA01_RS22865 (LLA01_22900)	4913696..4913800	-	105	WP_252173439.1	hypothetical protein	-
LLA01_RS22870 (LLA01_22905)	4914169..4915125	-	957	WP_252174357.1	ERCC4 domain-containing protein	-
LLA01_RS22875 (LLA01_22910)	4915282..4915803	+	522	WP_248689237.1	hypothetical protein	-
LLA01_RS22880 (LLA01_22915)	4915904..4916158	-	255	WP_252173440.1	Txe/YoeB family addiction module toxin	Toxin
LLA01_RS22885 (LLA01_22920)	4916155..4916403	-	249	WP_006550855.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LLA01_RS22890 (LLA01_22925)	4916499..4916819	+	321	WP_252173441.1	pirin-like C-terminal cupin domain-containing protein	-
LLA01_RS22895 (LLA01_22930)	4917234..4918028	+	795	WP_006550857.1	LLM class F420-dependent oxidoreductase	-
LLA01_RS22900 (LLA01_22935)	4918089..4919339	+	1251	WP_138845195.1	alpha/beta fold hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-70)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10139.46 Da
• Isoelectric Point: 8.0425

>T222480 WP_252173440.1 NZ_CP086069:c4916158-4915904 [Rhodococcus pyridinivorans]
VNITFTDEAWDDYQFWISTDRRQLKRVNELIRDCQRDPYSGIGKPERFRHALSGLWSRRIDKEHRLVYEVIDGALVIIAA
RYHY

Nucleotide

• Length: 255 bp

>T222480 NZ_CP086069:c4916158-4915904 [Rhodococcus pyridinivorans]
GTGAACATCACCTTCACCGACGAAGCCTGGGACGACTACCAGTTCTGGATCTCGACCGACAGAAGGCAGCTCAAGCGTGT
CAACGAGCTGATCAGGGATTGCCAACGAGACCCGTACTCGGGGATCGGCAAACCCGAACGGTTCAGACATGCCCTGTCAG
GACTGTGGTCTCGACGTATCGACAAAGAACACCGCCTGGTCTACGAGGTCATCGACGGTGCCCTGGTCATCATCGCCGCC
CGCTACCACTACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9290.59 Da
• Isoelectric Point: 5.8315

>AT222480 WP_006550855.1 NZ_CP086069:c4916403-4916155 [Rhodococcus pyridinivorans]
MAAITATEARKNFFPLLEQVNKDHTPVEIVSKRGNAVLMSKDDYDAMLETSYLMQSPANARRLLASMQRARGGEYTEREL
IE

Nucleotide

• Length: 249 bp

>AT222480 NZ_CP086069:c4916403-4916155 [Rhodococcus pyridinivorans]
ATGGCTGCCATCACTGCTACCGAAGCGAGAAAAAACTTCTTTCCGCTGCTCGAACAGGTCAACAAGGACCACACCCCTGT
GGAAATCGTATCCAAGCGGGGCAATGCCGTCCTGATGTCGAAGGATGACTACGACGCCATGCTCGAGACCTCGTACCTGA
TGCAGTCTCCGGCCAACGCCCGACGACTGCTCGCCAGCATGCAGAGAGCTCGCGGAGGTGAATACACCGAAAGGGAACTC
ATCGAGTGA

Structures

Antitoxin

• AlphaFold DB: H0JMW3