Detailed information of TA system TA222449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/COG3657-dnstrm_HI1420
• Location: 370532..371142
• Replicon: plasmid megaplasmid
• Accession: NZ_CP086019
• Organism: Ralstonia sp. RS642

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LGV80_RS19770
• Protein ID: WP_247394605.1
• Coordinates: 370532..370840 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LGV80_RS19775
• Protein ID: WP_016722339.1
• Coordinates: 370837..371142 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGV80_RS19750 (LGV80_19740)	366848..367447	-	600	WP_265087198.1	GNAT family N-acetyltransferase	-
LGV80_RS19755 (LGV80_19745)	367580..368131	+	552	WP_011003399.1	helix-turn-helix domain-containing protein	-
LGV80_RS19760 (LGV80_19750)	368309..369130	+	822	WP_011003398.1	GntR family transcriptional regulator	-
LGV80_RS19765 (LGV80_19755)	369257..370456	+	1200	WP_197343812.1	MFS transporter	-
LGV80_RS19770 (LGV80_19760)	370532..370840	+	309	WP_247394605.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LGV80_RS19775 (LGV80_19765)	370837..371142	+	306	WP_016722339.1	putative addiction module antidote protein	Antitoxin
LGV80_RS19780 (LGV80_19770)	371355..372668	+	1314	WP_247394606.1	MFS family transporter	-
LGV80_RS19785 (LGV80_19775)	372773..373468	-	696	WP_226951834.1	hypothetical protein	-
LGV80_RS19790 (LGV80_19780)	373608..373759	-	152	Protein_292	IS5/IS1182 family transposase	-
LGV80_RS19795 (LGV80_19785)	373886..374850	+	965	WP_265087199.1	IS5 family transposase	-
LGV80_RS19800 (LGV80_19790)	375330..375621	-	292	Protein_294	IS5/IS1182 family transposase	-
LGV80_RS19805 (LGV80_19795)	375891..376007	-	117	Protein_295	IS5/IS1182 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	flgG / flgC / cyaB / motA / cheW / cheB / cheY / adeG / acrB / fliI / fliG / fliM	1..1996921	1996921

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-88)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-96)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11836.59 Da
• Isoelectric Point: 6.7121

>T222449 WP_247394605.1 NZ_CP086019:370532-370840 [Ralstonia sp. RS642]
MLTIHTTEIFDDWFCSLRDKAAQRRIQVRIDRLQMGNPGDMKALRDGIRELRIDHGPGDRLYFVRHGVVLIVLLCGGDKS
TQETDIRRAIDLSRRLDVDDME

Nucleotide

• Length: 309 bp

>T222449 NZ_CP117857:526791-527141 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10967.57 Da
• Isoelectric Point: 7.2780

>AT222449 WP_016722339.1 NZ_CP086019:370837-371142 [Ralstonia sp. RS642]
MTMAQRTIKTRPWDSAEHLRTEADIAAYLDACLEEAGDDPAFITHALGVVARSRGMTQLARETGMTREGLYKALSEGGNP
SFATVLKVIRALGIRLHAVPT

Nucleotide

• Length: 306 bp

>AT222449 NZ_CP117857:526504-526785 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA