Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1430583..1431197 Replicon chromosome
Accession NZ_CP086016
Organism Pseudomonas aeruginosa isolate KB-PA_3

Toxin (Protein)


Gene name hicA Uniprot ID A6VBH9
Locus tag LKM46_RS06715 Protein ID WP_071534354.1
Coordinates 1430583..1430765 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A140SDX7
Locus tag LKM46_RS06720 Protein ID WP_012077229.1
Coordinates 1430793..1431197 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LKM46_RS06690 (LKM46_06685) 1426224..1426619 + 396 WP_003094374.1 hypothetical protein -
LKM46_RS06695 (LKM46_06690) 1426612..1427988 + 1377 WP_033970524.1 class II fumarate hydratase FumC -
LKM46_RS06700 (LKM46_06695) 1428016..1428465 + 450 WP_003094369.1 hypothetical protein -
LKM46_RS06705 (LKM46_06700) 1428478..1429089 + 612 WP_003098862.1 superoxide dismutase -
LKM46_RS06710 (LKM46_06705) 1429108..1430040 + 933 WP_079387315.1 ZIP family metal transporter -
LKM46_RS06715 (LKM46_06710) 1430583..1430765 + 183 WP_071534354.1 type II toxin-antitoxin system HicA family toxin Toxin
LKM46_RS06720 (LKM46_06715) 1430793..1431197 + 405 WP_012077229.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LKM46_RS06725 (LKM46_06720) 1431240..1432211 - 972 WP_012077228.1 hypothetical protein -
LKM46_RS06730 (LKM46_06725) 1432196..1432894 - 699 WP_033896049.1 hypothetical protein -
LKM46_RS06735 (LKM46_06730) 1432894..1433433 - 540 WP_012074127.1 hypothetical protein -
LKM46_RS06740 (LKM46_06735) 1433430..1434350 - 921 WP_012074128.1 hypothetical protein -
LKM46_RS06745 (LKM46_06740) 1434347..1435057 - 711 WP_012074129.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1430583..1468775 38192


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6702.74 Da        Isoelectric Point: 10.4826

>T222442 WP_071534354.1 NZ_CP086016:1430583-1430765 [Pseudomonas aeruginosa]
MRSREVIDLLLEDGWYEVAVKGSHHQFKHPSKPGKVTVQHPSSTIPKGTLNNILKQAGLK

Download         Length: 183 bp

>T222442 NZ_CP117853:c57079-56771 [Klebsiella michiganensis]
ATGCAGTATTACGTTTATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CAAGCGCAATACTCGGGTCGTAATTCCACTGTTTCCACTGAAAAACTATAAAGGTCCACGCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACATCGTGTTCTGGGTGAAGAA
GTTTGTAATCTGAATCACCAGCGCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14670.54 Da        Isoelectric Point: 4.4315

>AT222442 WP_012077229.1 NZ_CP086016:1430793-1431197 [Pseudomonas aeruginosa]
MKFPVVLHKDPDSDYGVTVPDVPGCFSAGATVSEALANVEEALALHFEGLVTDGEELPQPQDVDAHMKNPDFEGGVWAVV
DFDVTPYLGKAVRFNATLPEHLLQRIDERVKVDKRYQSRSGFLATAAMRELSVA

Download         Length: 405 bp

>AT222442 NZ_CP117853:c57318-57082 [Klebsiella michiganensis]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCAGGCCTGGTTAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCAGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A6VBH9

Antitoxin

Source ID Structure
AlphaFold DB A0A140SDX7

References