Detailed information of TA system TA222441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1314684..1315270
• Replicon: chromosome
• Accession: NZ_CP086016
• Organism: Pseudomonas aeruginosa isolate KB-PA_3

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: LKM46_RS06175
• Protein ID: WP_003120987.1
• Coordinates: 1314684..1314983 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: LKM46_RS06180
• Protein ID: WP_003448662.1
• Coordinates: 1314980..1315270 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKM46_RS06155 (LKM46_06150)	1309797..1310330	-	534	WP_003149521.1	type IV pilus biogenesis protein PilP	-
LKM46_RS06160 (LKM46_06155)	1310320..1311645	-	1326	WP_022579625.1	type 4b pilus protein PilO2	-
LKM46_RS06165 (LKM46_06160)	1311649..1313358	-	1710	WP_023093673.1	PilN family type IVB pilus formation outer membrane protein	-
LKM46_RS06170 (LKM46_06165)	1313358..1314482	-	1125	WP_023093672.1	TcpQ domain-containing protein	-
LKM46_RS06175 (LKM46_06170)	1314684..1314983	+	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LKM46_RS06180 (LKM46_06175)	1314980..1315270	+	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
LKM46_RS06185 (LKM46_06180)	1315353..1315688	-	336	WP_022579623.1	hypothetical protein	-
LKM46_RS06190 (LKM46_06185)	1315827..1317803	-	1977	WP_023093671.1	DEAD/DEAH box helicase	-
LKM46_RS06195 (LKM46_06190)	1317800..1319695	-	1896	WP_155713823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1254742..1345319	90577

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T222441 WP_003120987.1 NZ_CP086016:1314684-1314983 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T222441 NZ_CP117852:4515259-4515465 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATTCCCGAACTGCATCTTAAGGGCGACTGGCTGGCCGAGGCGGGATTTGAGACCGGGACGAGCGTAACCGTGAAGATCTC
GGAGGGGTGTTTGATCCTGATTGCAGAGACGGACGAAGTGAGGGATCTACGGAAGGAGCTTTATCTGGTGAAAAAGTCGA
TGAAGCATATTAAGGCGGGGGTAAATAATGTGGTGAACAGGGATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT222441 WP_003448662.1 NZ_CP086016:1314980-1315270 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT222441 NZ_CP117852:c4515455-4515270 [Salmonella enterica subsp. enterica serovar Mbandaka]
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCAGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCGTCCGTCTCTGCAATCAGGATCAAACACCCCTCCGAGATCTTCACGGTTACGCTCGTCCCGGTCTCAAATCCCGCCT
CGGCCAGCCAGTCGCCCTTAAGATGC

Structures

Toxin

• AlphaFold DB: G8CP73