Detailed information of TA system TA222285

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 92841..94111
• Replicon: chromosome
• Accession: NZ_CP085943
• Organism: Prevotella melaninogenica strain FDAARGOS_1566

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: LK441_RS07515
• Protein ID: WP_013265740.1
• Coordinates: 93176..94111 (+)
• Length: 312 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: -
• Locus tag: LK441_RS07510
• Protein ID: WP_013265071.1
• Coordinates: 92841..93176 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK441_RS07490 (LK441_07490)	88433..89539	+	1107	WP_044046024.1	MraY family glycosyltransferase	-
LK441_RS07495 (LK441_07495)	89873..90322	-	450	WP_013265542.1	lipocalin-like domain-containing protein	-
LK441_RS07500 (LK441_07500)	90356..91951	-	1596	WP_044046165.1	capsule assembly Wzi family protein	-
LK441_RS07505 (LK441_07505)	92641..92844	+	204	WP_013265868.1	helix-turn-helix transcriptional regulator	-
LK441_RS07510 (LK441_07510)	92841..93176	+	336	WP_013265071.1	HipA N-terminal domain-containing protein	Antitoxin
LK441_RS07515 (LK441_07515)	93176..94111	+	936	WP_013265740.1	HipA domain-containing protein	Toxin
LK441_RS07520 (LK441_07520)	94399..95985	-	1587	WP_013265909.1	fimbrillin family protein	-
LK441_RS07525 (LK441_07525)	96011..96172	-	162	WP_167310037.1	hypothetical protein	-
LK441_RS07530 (LK441_07530)	96951..97820	+	870	WP_013265448.1	hypothetical protein	-
LK441_RS07535 (LK441_07535)	98094..98612	-	519	WP_013265649.1	histidine phosphatase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (54-278)

Antitoxin

• HipA, N-terminal subdomain 1 (3-101)

Sequences

Toxin

Protein

• Length: 312 a.a.
• Molecular weight: 35897.58 Da
• Isoelectric Point: 8.2694

>T222285 WP_013265740.1 NZ_CP085943:93176-94111 [Prevotella melaninogenica]
MCKCLYCYQKLEEGQKDFHPHCARKFFGTKDVPLLEYKHKDLEELAEQVIRAQTSLTGVQPKLSLNLNKHDGCNRLTIVG
LWGDYIFKPQTEAYSQLPENEDLTMHLAEAAKIKVVPHSLIRLSDGKLGYLTKRIDRTKNGEKIDMEDMCQLTLHPTEYK
YKGSYEQIAKTITQYSSTPKLDLTNYMQLLLFCFITGNNDMHLKNFSLYRPSKDYQLAPAYDLLNTTIANPKDKEELAMP
LSGRKSKLCLDDFLNTAKTIGLEENIVLRLVKGFEKALPKWKLLIESSFLDEDMKKEYNKLIVSRLNRLQG

Nucleotide

• Length: 936 bp

>T222285 NZ_CP117750:986352-986873 [Enterobacter hormaechei]
GTGGCAAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTACGATTTCGGCGATTTCGATTGTGGTGAACCCTCGCT
CAATGCCTTCCTTACCGATCACCTGGTGCGTCAGCATGGCGGGCGTATTTTACGCGGCTACCTGCTTAAAGAACGCGACC
ATCCGCGTATCCTGGGCTATTACACCCTTTCGGGAAGTTGCTTTGAAAAAGCGATGCTTCCTTCAAAGACTCAGCAGCGC
CGCATCCCATACAGCAACGTCCCCAGCGTGACGCTGGGACGTCTGGCTGTCCATAAAGAACTACAGGGAAACGAGTGGGG
AACAACGCTTGTCACACACGCTATGCGCGTTGTTTATCTGGCGTCGCAGGCTGTTGGCGTCCACGGAATTTTTGTGGATG
CGCTTAACGAACGGGCCAAACGATTTTATCTGAAGCTGGGGTTTATCCCGCTGGCAGGTGAAAACAGCAGTTCGCTGTTC
TTCCCCACGCAATCTATCGAACGACTGTTTGAGCAGGCGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12583.68 Da
• Isoelectric Point: 4.3774

>AT222285 WP_013265071.1 NZ_CP085943:92841-93176 [Prevotella melaninogenica]
MRQAEIYRKRILAGILTEDGGEYRFCYDEKYLVQEDAQPISLTFPLQTEPFVSPVLFPFFDGLIPEGWLLDVALRNTNIS
VLDRMSLLLLCCKECIGAVSIIPRKELGGDV

Nucleotide

• Length: 336 bp

>AT222285 NZ_CP117750:986076-986345 [Enterobacter hormaechei]
ATGCCAGCACTCAAAAAACAGCGTATCGATCTTCGCCTGTCGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACCAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCCGAAGTCATCGAACAGCATCGGCGTC
TCGTATTAAACGAAGCCTCATGGAATGCCGTTATGGATGCCATCGACAATCCACCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA