Detailed information of TA system TA022192

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 3834951..3835636
• Replicon: chromosome
• Accession: NC_009525
• Organism: Mycobacterium tuberculosis H37Ra

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF49
• Locus tag: MRA_RS18105
• Protein ID: WP_003917170.1
• Coordinates: 3835226..3835636 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MRA_RS18100
• Protein ID: WP_003912220.1
• Coordinates: 3834951..3835229 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MRA_RS18080	3830940..3832541	-	1602	WP_003417969.1	FAD/NAD(P)-binding protein	-
MRA_RS18085	3832558..3833262	-	705	WP_003417980.1	dTDP-4-amino-4,6-dideoxyglucose formyltransferase	-
MRA_RS18090	3833380..3833946	-	567	WP_003417984.1	TetR/AcrR family transcriptional regulator	-
MRA_RS18095	3834008..3834895	+	888	WP_003900050.1	alpha-ketoglutarate-dependent sulfate ester dioxygenase	-
MRA_RS18100	3834951..3835229	+	279	WP_003912220.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MRA_RS18105	3835226..3835636	+	411	WP_003917170.1	type II toxin-antitoxin system VapC family toxin	Toxin
MRA_RS18110	3835669..3837405	-	1737	WP_003418002.1	cholesterol oxidase	-
MRA_RS18115	3837461..3838588	-	1128	WP_003418005.1	GuaB3 family IMP dehydrogenase-related protein	-
MRA_RS18120	3838608..3840197	-	1590	WP_003900682.1	IMP dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-54)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14669.76 Da
• Isoelectric Point: 5.1784

>T22192 WP_003917170.1 NC_009525:3835226-3835636 [Mycobacterium tuberculosis H37Ra]
VIYMDTSALTKLLISEPETTELRTWLTAQSGQGEDAATSTLGRVESMRVVARYGQPGQTERARYLLDGLDILPLTEPVIG
LAETIGPATLRSLDAIHLAAAAQIKRELTAFVTYDHRLLSGCREVGFVTASPGAVR

Nucleotide

• Length: 411 bp

>T22192 NC_009525:3835226-3835636 [Mycobacterium tuberculosis H37Ra]
GTGATCTATATGGACACCTCGGCCCTGACTAAGCTGCTCATCTCCGAGCCCGAGACGACCGAACTGCGGACATGGCTGAC
CGCGCAAAGCGGCCAGGGCGAGGACGCGGCGACAAGCACCCTTGGCCGGGTCGAGTCGATGAGAGTCGTTGCCCGATACG
GACAACCAGGCCAAACTGAGCGTGCGCGTTACCTACTCGACGGGCTCGACATCCTCCCGCTCACCGAACCGGTGATCGGT
CTAGCTGAAACGATCGGACCGGCCACCCTACGTTCTCTCGACGCGATTCACCTCGCGGCCGCAGCCCAGATCAAGCGGGA
ACTGACAGCCTTCGTCACCTACGACCACCGATTGTTGAGCGGATGCCGTGAGGTCGGCTTCGTCACCGCCTCACCCGGCG
CAGTCCGGTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10280.75 Da
• Isoelectric Point: 10.1819

>AT22192 WP_003912220.1 NC_009525:3834951-3835229 [Mycobacterium tuberculosis H37Ra]
VEAIGIRELRQHASRYLARVEAGEELGVTNKGRLVARLIPVQAAERSREALIESGVLIPARRPQNLLDVTAEPARGRKRT
LSDVLNEMRDEQ

Nucleotide

• Length: 279 bp

>AT22192 NC_009525:3834951-3835229 [Mycobacterium tuberculosis H37Ra]
GTGGAGGCAATCGGAATCCGAGAACTAAGACAGCACGCATCGCGATACCTCGCCCGGGTTGAAGCCGGCGAGGAACTTGG
CGTCACCAACAAAGGAAGACTTGTGGCCCGACTCATCCCGGTGCAGGCCGCGGAGCGTTCTCGCGAAGCCCTGATTGAAT
CAGGTGTCCTGATTCCGGCTCGTCGTCCACAAAACCTTCTCGACGTCACCGCCGAACCGGCGCGCGGCCGCAAGCGCACC
CTGTCCGATGTTCTCAACGAAATGCGCGACGAGCAGTGA

Structures

Toxin

• AlphaFold DB: A5U883